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Cell image analysis method and cell image analysis device

An image analysis and cell technology, which is applied in the field of cell image analysis and cell image analysis devices, can solve problems such as heavy user burden, and achieve the effect of reducing user burden

Pending Publication Date: 2022-03-01
SHIMADZU SEISAKUSHO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Such a cell search operation and selection operation are delicate and the burden on the user is heavy, so a device that automates a part of the operation has been proposed (for example, refer to Non-Patent Document 1).

Method used

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  • Cell image analysis method and cell image analysis device
  • Cell image analysis method and cell image analysis device
  • Cell image analysis method and cell image analysis device

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Experimental program
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Embodiment Construction

[0036] Hereinafter, embodiments embodying the present invention will be described based on the drawings.

[0037] refer to Figure 1 to Figure 14 The cell image analysis method and the cell image analysis device 100 of the present embodiment will be described.

[0038] (Cell image analysis method)

[0039] figure 1 The cell image analysis method shown is a method of acquiring a microscope image obtained by photographing a cell colony 10 in the selection operation of cells in cell culture, and determining whether or not the cell colony 10 captured in the microscope image includes a candidate to be searched. colony area.

[0040] A cell colony 10 is a cell mass (collection of a plurality of cells) derived from a single cell. A colony region is a region composed of specific cells in a cell colony.

[0041] The selection of cells refers to extracting cells or cell clusters to be selected from the cell culture container 90 . For example, cells are selected by aspirating the s...

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Abstract

The cell image analysis method comprises the steps of: converting a first image (21) into a tag image (21A) by performing a segmentation process for specifying a region (12) of cells in which differentiation has begun and a region (11) of undifferentiated cells in the first image (21); acquiring a shape feature quantity (25) from the label image; and determining, on the basis of the shape feature amount (25) and a determination reference (30), whether or not a candidate colony region to be searched is included.

Description

technical field [0001] The invention relates to a cell image analysis method and a cell image analysis device. Background technique [0002] Conventionally, a technique for culturing pluripotent cells such as iPS cells (induced pluripotent stem cells: induced pluripotent stem cells) is known. [0003] Passaging is carried out during cell culture, and in this passaging, cells in culture are extracted from a culture vessel and transferred to another culture vessel to be used as a next-generation cell line. The extraction of the cells is called picking. Cells that are not suitable for passage, such as cells that have already begun to differentiate (cells that have lost pluripotency), exist in the culture vessel. Therefore, the operator (user) selects cells suitable for passage during passage. [0004] Specifically, a worker (user) checks the cell colonies in the culture container one by one with a microscope to search for cells suitable for passage in the culture container. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12M1/34G06T7/00
CPCG06T7/00C12Q1/04C12M1/34G06V10/70G06V20/69G06V20/695G06V10/235G06V10/774G06V10/46
Inventor 高桥涉
Owner SHIMADZU SEISAKUSHO CO LTD