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Polypeptide repair liquid

A technology for repairing liquid and polypeptide, applied in the field of polypeptide repairing liquid, can solve problems such as toxic and side effects, and achieve the effects of reducing toxic and side effects, reducing the dosage, and preventing excessive oxidation.

Pending Publication Date: 2022-03-18
北海添亮生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, with the in-depth research on the activity of nano-silver, people found that at a higher level, nano-silver will produce certain toxic and side effects in the body (Braydich-Stolle LK et al. (2010).Silver nanoparticles disrupt GDNF / Fyn kinase signaling in spermatogonial stem cells.Toxicol Sci116(2):577-589.);(Hu XK et al.(2020).Fructose-coated Angstrom silver inhibitsosteosarcoma growth and metastasis via promoting ROS-dependent apoptosis through the alteration of glucose metabolism by PDK.inhibiting Theranostics10 (17): 7710-7729.), so the dosage of nano-silver must be controlled in an appropriate range during use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of Example 1 Polypeptide Repair Solution

[0037] (1) Preparation of pearl hydrolyzed active peptide solution by enzymatic hydrolysis

[0038] Pearls (from Pinctada martensii) from marine culture in the Beibu Gulf are ground into fine powders of 200-270 mesh, then added with distilled water, stirred to make an aqueous solution containing 50-60% (g / v) of pearl powder, and then the pearls Heat the powder aqueous solution and keep it at 45-58°C, add neutral protease (40000-50000U / G) and compound protease (10000-20000U / G) with pearl powder weight 1‰-5‰, and keep stirring for 4-5 hours Filter out the solid matter to obtain the enzymolyzed pearl hydrolyzed active polypeptide solution, then put the enzymatically hydrolyzed pearl hydrolyzed active polypeptide solution into a distillation dish, heat to 95-100°C for 5-15 minutes, and finally collect Distillate.

[0039] (2) Prepare Bingtangcao lavender extract

[0040] Purchasing the petals of high-quality, non-rot...

Embodiment 2

[0049] The bacteriostasis effect comparison of each component of embodiment 2 polypeptide repair liquid

[0050] Because the peptide repair solution contains nano-silver, lavender rock sugar grass extract and other ingredients, we first carried out a preliminary evaluation of the antibacterial activity of these ingredients. The 12 wells in each row of the 96-well plate are numbered 1 to 12 respectively. Well 1 is a blank control, well 2 is a negative control, wells 3 and 4 are positive drug control wells with different concentrations, and wells 5-7 are peptides. Repair liquid stock solution, 50% dilution of the stock solution, and 10% dilution of the stock solution. Wells 8 to 12 are pearl hydrolyzate, lavender rock sugar grass extract, nano silver, gourd tea extract, and Prunella vulgaris extract. Their respective concentrations are the same as those in the polypeptide repair solution. The total volume after dilution in the culture medium is 100 μl, and then 100 μl of culture...

Embodiment 3

[0052] Example 3 The detection of the inhibitory activity of the polypeptide repair solution to 8 strains to be tested

[0053] (1) Using the double dilution method, the peptide repair solution was treated with Pseudomonas aeruginosa, Staphylococcus epidermidis, Klebsiella pneumoniae, Staphylococcus aureus, Propionibacterium acnes, Actinomyces viscosus, Porphyromonas gingivalis Bacteria and 8 strains of Acinetobacter baumannii were serially diluted (after the strains were resuscitated and activated, a single clone was picked and inoculated in the corresponding medium, and when the OD600 value of the bacterial solution reached 0.2-0.4, the 1:100 (v / v) dilution to prepare the inoculum solution), a total of 8 concentrations: 100% stock solution, 50% stock solution, 40% stock solution, 30% stock solution, 20% stock solution, 10% stock solution, 5% stock solution and 2.5% stock solution.

[0054] (2) Use a pipette gun to add 100 μl each of the prepared double-diluted 8 concentrati...

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Abstract

The invention discloses a polypeptide repair liquid, which is characterized in that pearl hydrolysate, lavender, sweet broomwort herb, triquetrous tadehagi herb, prunella vulgaris and other natural plant extracts are adopted to prepare a novel burn and scald nursing preparation with repair and bacteriostasis functions through an ultrasonic process. Natural plants such as lavender and selfheal are rich in various antibacterial active substances, and can exert synergistic antibacterial activity and enhance the antibacterial effect when being combined with the nano-silver simple substance, so that the dosage of the nano-silver is reduced, and the toxic and side effects of the nano-silver are reduced and avoided. The triquetrous tadehagi herb extract and the selfheal extract are rich in immunoregulation active substances and antioxidant active substances, can assist skin injury repair, can also provide a reductive microenvironment for the nano-silver simple substance and prevent the nano-silver from being excessively oxidized, so that the action time of the antibacterial liquid is prolonged, the product cost can be further reduced, and the product quality is improved. And the toxic and side effects of nano-silver are reduced.

Description

technical field [0001] The invention belongs to the technical field of biological products, and in particular relates to a polypeptide repair solution. Background technique [0002] The skin is the largest organ of the human body, and it is also the first natural barrier of the body's immune protection. Once it is severely damaged by the outside world and loses its immune and protective functions, it will cause a series of stress reactions in the human body, resulting in shock , infection and other complications, even life-threatening. Burns and scalds are one of the more common skin injuries in people's daily life. The skin wounds left by burns and scalds not only cause physical pain to people, but the skin scars caused by improper treatment affect the beauty of the face and body, and also create mental pressure on people. . [0003] For mild burns and scalds, the skin relies on its own deep dermal cells to complete its own repair, and generally no scars are left. When t...

Claims

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Application Information

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IPC IPC(8): A61K8/98A61K8/64A61K8/9789A61K8/19A61Q19/00A61Q17/00
CPCA61K8/987A61K8/64A61K8/9789A61K8/19A61Q19/00A61Q17/005A61K2800/805A61K2800/5922A61K2800/82A61K2800/413
Inventor 唐振洲顾勇彪高程海杨俊甘雨满罗双宇
Owner 北海添亮生物技术有限公司
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