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Lactobacillus plantarum P101 with high antioxidant activity and application thereof

A technology of Lactobacillus plantarum and oxidation activity, which is applied to Lactobacillus plantarum P101 with high antioxidant activity and its application fields, and can solve the problem of low hydroxyl radical scavenging rate of Lactobacillus plantarum, intolerant gastrointestinal extreme environment, gastrointestinal Weak tolerance and other problems, to achieve good acid tolerance and bile salt tolerance, alleviate alcoholic liver injury, and good antibacterial activity

Active Publication Date: 2022-03-18
NANCHANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, studies have reported many strains of Lactobacillus plantarum with antioxidant activity, but there are generally disadvantages such as poor antibacterial effect or intolerance to the extreme environment of the gastrointestinal tract.
Among them, Chinese patent CN109182162A provides a plateau plantarum lactobacillus, which has certain antioxidant activity and can effectively remove free radicals in the body, but has a low survival rate in intestinal juice and weak gastrointestinal tolerance; Chinese patent CN109182162A provides a strain of Lactobacillus plantarum with antioxidant activity, its preservation number is CGMCC15780, and the hydroxyl radical scavenging rate of Lactobacillus plantarum is low

Method used

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  • Lactobacillus plantarum P101 with high antioxidant activity and application thereof
  • Lactobacillus plantarum P101 with high antioxidant activity and application thereof
  • Lactobacillus plantarum P101 with high antioxidant activity and application thereof

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Experimental program
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Embodiment

[0029] 1 Isolation and purification of lactic acid bacteria

[0030] 1.1 Isolation of lactic acid bacteria

[0031] Take 200 μL of fermented vegetable samples from fermented vegetable samples made by farmers in Ji’an, Jiangxi, and enrich and culture them in MRS liquid medium for 24 hours, then dilute the culture solution with sterile PBS according to the concentration gradient, and draw 100 μL to evenly spread it to a concentration of 1% calcium carbonate. On the modified MRS solid medium, each concentration was repeated twice. Place it in a constant temperature incubator at 37°C for 48 hours to observe the formation of colonies and transparent circles, which is convenient for further separation and purification.

[0032] 1.2 Purification culture

[0033] Use an inoculation loop to pick a single colony with a clearly visible border and a transparent circle, streak it in the MRS solid medium, and place the plate in an anaerobic incubator at 37°C for 24-48h. After streaking 3...

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Abstract

The invention discloses a lactobacillus plantarum P101 with high antioxidant activity and application thereof, the lactobacillus plantarum P101 is separated from farmhouse homemade pickled Chinese cabbages in Jiyan, Jiangxi Province, the 16S rRNA sequence of the lactobacillus plantarum P101 is shown as SEQ ID NO: 1, and the preservation number is CCTCC M 2021108. The lactobacillus plantarum P101 disclosed by the invention has good antibacterial activity and can be used for remarkably inhibiting the activity of enteropathogenic bacteria such as staphylococcus aureus and pseudomonas aeruginosa; the lactobacillus plantarum P101 has high antioxidant activity, in-vitro experimental results show that the clearance rate of the lactobacillus plantarum P101 to 1, 1 diphenyl-2-trinitrophenylhydrazine (DPPH) and hydroxyl radicals is remarkably higher than that of lactobacillus rhamnosus GG (LGG), and in-vivo experimental results show that the lactobacillus plantarum P101 can effectively weaken oxidative stress injury induced by alcohol and inhibit inflammatory response of the organism. The survival rate of the lactobacillus plantarum P101 in an acid or high cholate environment is obviously higher than that of lactobacillus plantarum ZDY2013, and in addition, the lactobacillus plantarum P101 has outstanding benefits in relieving fatty liver hepatitis induced by alcohol.

Description

technical field [0001] The invention belongs to the field of microbial technology and food fermentation, and in particular relates to a strain of Lactobacillus plantarum P101 with high antioxidant activity and its application. Background technique [0002] Oxidative stress refers to a state of imbalance between oxidation and anti-oxidation in the body. When the body is in a state of oxidative stress, the reactive oxygen species (ROS) accumulated in the body will cause free radical chain reactions by destroying biomolecules. Excessive free radicals It will reduce the body's resistance, reduce the cell renewal rate, induce oxidative damage, cause inflammation and aging, etc. Oxidative stress is a major predisposing factor for many diseases, such as: cardiovascular and cerebrovascular diseases, liver diseases and cancer. When the body's oxidation and anti-oxidation are out of balance, the content of free radicals and malondialdehyde (MDA) increases, and the activity of anti-ox...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P31/04A61P37/02A61P39/06A61P1/16A23L33/135A23L29/00C12R1/25
CPCC12N1/20A61K35/747A61P31/04A61P37/02A61P39/06A61P1/16A23L33/135A23L29/065A23V2002/00A23V2400/169A23V2200/3204A23V2200/324A23V2200/334A23V2200/30Y02A50/30
Inventor 许恒毅刘善级胥晓薇王梦琦胡烈海
Owner NANCHANG UNIV
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