Electrochemical heavy metal rapid detection method based on electrically-driven pre-enrichment

A detection method and electrochemical technology, applied in the field of biosensors, can solve the problems of long incubation time and influence, and achieve the effects of good stability, good selectivity and improved selectivity

Active Publication Date: 2022-03-18
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Electrochemical aptasensor in Hg 2+ It has broad prospects in the selectivity analysis; Wu Danhong et al. can specifically capture Hg by using T-T mismatch 2+ , with T-Hg 2+ -T forms a DNA duplex, however, T-Hg 2+ -T structure formation is still affected by longer incubation times

Method used

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  • Electrochemical heavy metal rapid detection method based on electrically-driven pre-enrichment
  • Electrochemical heavy metal rapid detection method based on electrically-driven pre-enrichment
  • Electrochemical heavy metal rapid detection method based on electrically-driven pre-enrichment

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Embodiment 1

[0043] (1) Manufacturing process of electrostatic field sensing biosensor:

[0044] The present invention also provides a pressure device for generating an electrostatic field; the device is composed of two parallel metal plates and a constant voltage drive device controlled by a single-chip microcomputer,

[0045] An electrode frame is arranged between the two parallel metal plates, and the electrode frame is used to place Hg 2+ Standard solution and electrode;

[0046] The constant voltage driving device is composed of a constant voltage power supply, DAC0832 and STC89C52; the constant voltage power supply is electrically connected to the DAC0832 and STC89C52;

[0047] The metal plate is connected with the output port of DAC0832 to realize voltage output and conversion, and an electrostatic field area will be generated between the two metal plates. Supply power to DAC0832 and STC89C52 through the power supply, the specific structure is as follows Figure 4 shown.

[0048...

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Abstract

The invention belongs to the technical field of biosensors, and relates to an electrochemical heavy metal rapid detection method based on electrically-driven pre-enrichment. The method comprises the following steps: firstly, polishing a gold electrode with aluminum oxide powder, performing ultrasonic treatment in ethanol and water, and drying to obtain a treated gold electrode; modifying an Fc-labeled Hg < 2 + > aptamer (Fc-apt) on the surface of the treated gold electrode, and recording as Fc-apt / AuE; then modifying MCH on the surface, naturally airing at room temperature, and recording as MCH / Fc-apt / AuE; and finally, incubating the Hg < 2 + > by using an electrostatic field device, applying 0.5-2.5 V voltage to pre-enrich the Hg < 2 + >, and applying-0.5--2.5 V voltage to enable the surface of the electrode to achieve a self-cleaning effect. The electrochemical biosensor prepared by the invention has the advantages of rapid detection, pre-enrichment, high sensitivity, self-cleaning and the like, is a sensor for integrally detecting Hg < 2 + >, and is used for sensitively and rapidly analyzing an actual sample.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and in particular relates to an electrochemical heavy metal rapid detection method based on electric drive pre-enrichment. Background technique [0002] Mercury (Hg 2+ ) is listed by the World Health Organization (WHO) as the most dangerous heavy metal pollutant due to acute toxicity, non-biodegradable nature, and high bioaccumulation in ecosystems. Therefore, the rapid determination of mercury with high sensitivity and selectivity is very important in environmental protection, because the presence of mercury, even at trace levels, can be absorbed by plants and animals, and further biochemicalized in the human body through the food chain, causing neurological damage. System and organs cause certain damage. So far, several Hg 2+ The detection method, the Hg that has been established at home and abroad 2+ The detection methods include inductively coupled plasma mass spectrometry (ICP-MS), at...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): G01N27/49
CPCG01N27/49Y02A50/30
Inventor刘东刘畅李玉叶陈婷由天艳
OwnerJIANGSU UNIV