Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for quantifying diaminopimelic acid-containing bacteria

A technology of diaminopimelic acid content and diaminopimelic acid, which is applied in the field of quantification of bacteria containing diaminopimelic acid, can solve the problem that the number of bacteria cannot be measured by culture method

Pending Publication Date: 2022-03-22
SUNTORY HLDG LTD
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] On the other hand, in the case of powder of dead bacteria or products added with it, since the added bacteria are dead bacteria, the number of bacteria cannot be measured by culture method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for quantifying diaminopimelic acid-containing bacteria
  • Method for quantifying diaminopimelic acid-containing bacteria
  • Method for quantifying diaminopimelic acid-containing bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0133] In the sample prepared in Example 1, the bifidobacteria raw material was contained other than the lactic acid bacteria S-PT84. Confirm the amount of DAP for bifidobacteria raw materials. Results No DAP was detected from the bifidobacterium raw material.

[0134] As for the raw material of lactic acid bacteria S-PT84, the bacterial cells are cultured on a culture medium (containing yeast extract and glucose), separated from solid and liquid, and the precipitated part is freeze-dried and pulverized to be raw material. In consideration of the medium being mixed with this raw material, the confirmation of the amount of DAP was implemented about the medium raw material of lactic acid bacteria S-PT84. Results DAP was not detected from any sample. From the above results, it is considered that in Example 1, all the DAPs detected in the samples come from lactic acid bacteria S-PT84.

[0135] From the above results, it can be seen that the number of bacteria containing DAP can...

Embodiment 2

[0137] Bifidobacterium bifidum (B. bifidum) NBRC100015 strain (hereinafter referred to as Bifidobacterium NBRC100015) of bifidobacteria was used as the bacteria not containing DAP. Bifidobacterium NBRC100015 can be obtained from the Bioengineering Center of the independent administrative agency Product Evaluation Technology Foundation (NBRC No.NBRC 100015). In order to mix with the raw material of lactic acid bacteria S-PT84, add skimmed powdered milk to the culture solution of Bifidobacterium NBRC100015 to prepare powdered Bifidobacterium NBRC100015 raw material (3.4×10 10 pieces / g containing products). In addition, the viable count of the bifidobacterium NBRC100015 raw material is a measured value based on the Japan Food Analysis Center of the General Incorporated Foundation. The raw material of lactic acid bacteria S-PT84 uses the dead bacteria powder of the same lactic acid bacteria S-PT84 as Test Example 2 (manufactured by Dayang Fragrance Co., Ltd., dead bacteria 1.8×10...

Embodiment 3

[0143] As DAP-free bacteria, lactic acid bacteria Lactobacillus acidophilus (L. acidophilus) NBRC13951 strain (hereinafter referred to as lactic acid bacteria NBRC13951) was used. Lactic acid bacteria NBRC13951 can be obtained from the Bioengineering Center of the independent administrative agency product evaluation technology foundation institution (NBRC No. NBRC 13951). In order to mix with the raw material of lactic acid bacteria S-PT84, add skimmed powdered milk to the culture solution of lactic acid bacteria NBRC13951 to prepare powdered raw materials of lactic acid bacteria NBRC13951 (live bacteria 1.0×10 8 pieces / g containing products). In addition, the viable count of the lactic acid bacterium NBRC13951 raw material is a measured value based on the Japan Food Analysis Center of the General Incorporated Foundation.

[0144] The raw material of lactic acid bacteria S-PT84 uses the dead bacteria powder of the same lactic acid bacteria S-PT84 as Test Example 2 (manufactur...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The purpose of the present invention is to provide a novel method with which it is possible to quantify diaminopimelic acid-containing bacteria contained in a test sample. The present invention relates to a method for quantifying diaminopimelic acid-containing bacteria contained in a sample to be detected, the method being characterized by comprising: determining the amount of diaminopimelic acid derived from the diaminopimelic acid-containing bacteria in the sample to be detected as an index, and determining the amount of diaminopimelic acid derived from the diaminopimelic acid-containing bacteria in the sample to be detected; and a step for quantifying the bacteria containing diaminopimelic acid.

Description

technical field [0001] The present invention relates to a method for quantifying diaminopimelic acid-containing bacteria contained in a test sample. Background technique [0002] Addition of lactic acid bacteria and the like to food is carried out due to the improvement of the will to be healthy. Such bacteria may be added as living bacteria (live bacteria), or may be added as dead bacteria (dead bacteria). For example, when formulating bacteria such as lactic acid bacteria as functionally related ingredients in functionally labeled foods, it is important to determine the number of live bacteria and dead bacteria contained in the product. [0003] As a method for quantifying viable bacteria contained in a sample, a method of measuring colonies after culturing using an agar medium (cultivation method) and the like are used. For example, in Patent Document 1, a method for measuring the number of viable bacteria is described, which is characterized in that, as a method for me...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/06
CPCC12Q1/06C12R2001/25C12R2001/225C12N1/205
Inventor 佐藤洋介笠岛直树福田俊彦井田正幸
Owner SUNTORY HLDG LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com