A biological preparation for improving the fermentation quality of mixed silage and its preparation method

A technology of mixing silage and biological preparations, applied in the preservation method of animal feed raw materials, bacteria and animal feed used in food preparation, etc., can solve the problems of low digestion and utilization rate, poor palatability, etc. The effect of reducing ammonia content and inhibiting secondary fermentation

Active Publication Date: 2022-05-13
SHANDONG JIANYUAN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the existence of anti-nutritional factors, untreated woody feed directly fed to livestock has poor palatability and low digestibility, so silage is required.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: the preparation of mulberry leaf extract

[0038]a. Crush 100g of mulberry leaves through a 20-mesh sieve, mix with 750mL of water and cook for 0.5h to obtain mulberry leaf liquid, take 200g of mulberry leaf liquid to cool, add 4g of cellulase and 4g of pectinase to hydrolyze at 50°C for 18h, and filter to obtain enzymatic solution;

[0039] b. Concentrate the enzymolysis solution in vacuum at 65°C with a vacuum degree of 80 kpa, and obtain the extract after cooling. The extract was chromatographed on a polyamide column (diameter-to-height ratio 1:10), and ethanol-ethyl acetate with a volume ratio of 4:1 was used as the eluent, and the amount of eluent was 3 times the volume of the column. The eluate was collected, concentrated, and spray-dried to obtain the mulberry leaf extract.

Embodiment 2

[0040] Embodiment 2: the preparation of Jerusalem artichoke extract

[0041] a. Pulverizing and drying the Jerusalem artichoke tubers until the water content is 8wt% to obtain the Jerusalem artichoke granules;

[0042] b. Add 1.5L of ethanol with a volume fraction of 80% to 100g of Jerusalem artichoke granules, heat to 70°C for reflux extraction for 40min, cool and filter the obtained liquid, concentrate and spray dry to obtain Jerusalem artichoke extract.

Embodiment 3

[0044] (1) Streak the lines of Lactobacillus brucei CICC 20293 and Lactobacillus plantarum CICC 20765 on the MRS solid slant respectively, cultivate colonies of Lactobacillus brucei at 37°C and Lactobacillus plantarum at 35°C, activate twice and pick Take a single colony and inoculate it in the MRS liquid medium, shake the flask overnight (100rpm / min) to obtain the seed solution of Lactobacillus brucei and Lactobacillus plantarum, and the bacterial concentration of the seed solution of Lactobacillus brucei and Lactobacillus plantarum Adjust to 1.0×10 9 CFU / mL.

[0045] (2) Take 1L of MRS liquid medium, add 80g of the mulberry leaf extract prepared in Example 1, and 150g of the Jerusalem artichoke extract prepared in Example 2, mix evenly to obtain a fermentation medium, and divide the fermentation medium into 2 parts. Inoculate 50 mL of Lactobacillus brauchneri seed solution and 50 mL of Lactobacillus plantarum seed solution into the fermentation medium, ferment at 36° C. for...

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PUM

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Abstract

The invention discloses a biological preparation for improving the fermentation quality of mixed silage and a preparation method thereof, belonging to the field of silage additives. The method is as follows: (1) respectively culturing Lactobacillus brucei and Lactobacillus plantarum to obtain Lactobacillus brucei seed liquid and Lactobacillus plantarum seed liquid; The method is inoculated into fermentation media containing mulberry leaf extract and Jerusalem artichoke extract respectively for cultivation, and is filtered to obtain Lactobacillus brauchneri fermentation broth and Lactobacillus plantarum fermentation broth, which are mixed to obtain a highly active biological agent. In the present invention, the mulberry leaf extract and the Jerusalem artichoke tuber extract are added to the culture medium, and the fermentation bacteria are cultivated to obtain the highly active biological preparation. After use, the fermentation speed is fast, which can quickly generate lactic acid, effectively inhibit the growth of spoilage bacteria, prevent forage from mold and rot; enhance the palatability of silage, and increase the content of crude protein, organic acids, vitamins, and amino acids.

Description

technical field [0001] The invention relates to the technical field of silage additives, in particular to a biological preparation for improving the fermentation quality of mixed silage and a preparation method thereof. Background technique [0002] At present, there are three main methods of silage processing straw at home and abroad. One is ammonification treatment. Chemical fertilizers with high nitrogen content such as ammonium bicarbonate and urea are added to the straw for ammoniation to inhibit the activity of microorganisms to achieve the purpose of freshness. This method The advantage is that it can keep the silage fresh and increase the non-protein nitrogen of the silage at the same time, but the disadvantage is that the pH value is high, and it is easy to be infected with bacteria and deteriorate after being exposed to the air, and the excessively high non-protein nitrogen is easy to produce melamine when encountering high temperature during the silage fermentation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/30A23K10/12A23K30/18
CPCA23K10/30A23K10/12A23K30/18A23V2400/11A23V2400/169
Inventor 王诚董桂红何荣彦李燕赵福平王玲巩倩邢烛宗倩刘雨董晓桐
Owner SHANDONG JIANYUAN BIOTECHNOLOGY CO LTD
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