Bioremediation method for heavy metal contaminated soil
A bioremediation and contaminated soil technology, applied in the field of bioremediation of heavy metal-contaminated soil, can solve the problems of single planting, less complex heavy metal pollution, and single heavy metal to be repaired, and achieve the elimination of empty window period, remarkable repair effect and huge application potential Effect
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Embodiment 1
[0030] The test site is a cadmium-lead compound polluted farmland in Sidi Village, Yangshuo County, Guilin City, Guangxi. The cause of the pollution is the downstream farmland pollution caused by the upstream lead-zinc mine tailings. The soil physical and chemical properties: total cadmium 2.70mg / kg, effective cadmium 1.52mg / kg kg, total lead 275mg / kg, available lead 139mg / kg, pH 6.11, organic matter content 0.5%, ammonium nitrogen 19.3mg / kg, available phosphorus 53.1mg / kg, available potassium 213.6mg / kg.
[0031] The test area was divided into 1 control group and 3 treatment groups:
[0032] The control group was not planted;
[0033] Treatment group: Crop rotation of Taiwan green sedum and German sedum (crop rotation group), Taiwan green sedum and German sedum alone;
[0034] Each group was repeated 3 times, each with an area of 2m×4m, and all plots were randomly arranged to control the field experiment error.
[0035] Control group: During the entire test period, no pla...
Embodiment 2
[0048] The heavy metal-enriched plants Pseudomonas taiwanensis and Sedum germanum were used as plant materials. The seeds of Taiwan sage are taken from the demonstration base of phytoremediation of heavy metal pollution in the school, which is located in the tailings bank of the lead-zinc mine in Sidi Village, Xingping Town, Yangshuo County, Guilin City. Select seeds with full grains and use 10% H 2 o 2 After soaking and disinfecting the solution, rinse with distilled water and sow seeds in the seedling trays, place the seedling trays in a greenhouse, keep the temperature at 25-30°C during the day, 15-20°C at night, and the light intensity at 250μmolphotons m -2 the s -1 , The average daily light time is 12-14 hours, and the relative humidity is 70-75%. Irrigate with tap water (Cd not detected) every day, and keep the soil water content at 50%. After the seeds germinate, irrigate with 1 / 2 concentration of Hongland nutrient solution once a week. After the seedlings grow 4 t...
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