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System and method for evaluating sample fixation duration and quality using vibration spectroscopy

A fixed-duration, vibrational spectrum technology, used in color/spectral property measurement, neural learning methods, measurement devices, etc., to solve problems such as insufficient staining and antigen retrieval conditions that do not function properly

Pending Publication Date: 2022-04-12
VENTANA MEDICAL SYST INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tissues exposed to formalin for prolonged periods of time generally do not perform well for subsequent immunohistochemical procedures, presumably due to nucleic acid and / or protein denaturation and degradation
As a result, optimal antigen retrieval conditions for these tissues are not functioning properly, so tissue samples appear to be understained

Method used

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  • System and method for evaluating sample fixation duration and quality using vibration spectroscopy
  • System and method for evaluating sample fixation duration and quality using vibration spectroscopy
  • System and method for evaluating sample fixation duration and quality using vibration spectroscopy

Examples

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example 1

[0155] Example 1 - Predicting a fixed duration using a trained fixed estimation engine

[0156] The examples provided below illustrate the methods described in this article.

[0157] introduce

[0158] Modern histology builds on the more than 100-year-old cornerstone technology of formaldehyde fixation, which preserves the biological structure of tissue by cross-linking mechanisms that prevent molecular degradation. The thoroughness of sample fixation can significantly affect the detected protein expression and, in extreme cases, the patient's diagnosis. For example, ASCO / CAP guidelines call for samples to be fixed for 6 to 72 hours. Tissue fixation in current clinical practice is laboratory-specific and highly variable. Despite its importance, there is no analytical method for measuring a fixed mass.

[0159] Mid-infrared spectroscopy (mid-IR) is a powerful optical technique for probing the vibrational states of individual molecules in tissues and is very sensitive to...

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Abstract

The present disclosure relates to an automated system (200) and method for quantitatively determining a fixed duration of a biological sample using a trained fixed estimation engine (210). In some embodiments, a trained fixed estimation engine (210) includes a neural network. In some embodiments, the trained fixed estimation engine (210) includes a supervised classifier.

Description

[0001] Cross references to related patent applications [0002] This application claims the benefit of the filing date of U.S. Patent Application No. 62 / 892,678, filed August 28, 2019, the disclosure of which is hereby incorporated by reference in its entirety. Background technique [0003] Immunohistochemical (IHC) slide staining can be used to identify proteins in cells of tissue sections and is therefore widely used to study different types of cells, such as cancer cells and immune cells in biological tissues. Therefore, IHC staining can be used to study the distribution and localization of biomarkers differentially expressed by immune cells (such as T cells or B cells) in cancer tissues for immune response research. For example, tumors often contain an infiltrate of immune cells, which may prevent tumor development or promote tumor growth. [0004] In situ hybridization (ISH) can be used to determine the presence of genetic abnormalities or conditions such as specific amp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B40/00
CPCG01N21/35G01N21/65G01N2201/1296G01N2201/1293G01N2021/399G01N21/3563G01N21/39G01N33/4833G01N2201/06113G06N3/08G01N1/30G01N21/1702
Inventor D·鲍尔D·查芬
Owner VENTANA MEDICAL SYST INC