Antibody, preparation method thereof, coding nucleic acid and engineering cell

A technology of antibodies and B cells, applied in botany equipment and methods, biochemical equipment and methods, genetic engineering, etc., can solve the problems of limited plasma sources and cannot be widely used, and achieve high sensitivity and excellent pathogen neutralization effect , good effect of antibody specificity

Pending Publication Date: 2022-04-15
蒋庆华 +3
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, although the treatment with plasma from recovered patients is

Method used

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  • Antibody, preparation method thereof, coding nucleic acid and engineering cell
  • Antibody, preparation method thereof, coding nucleic acid and engineering cell
  • Antibody, preparation method thereof, coding nucleic acid and engineering cell

Examples

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Embodiment 1

[0049] This example is used to illustrate the process of the antibody screening method of the present disclosure.

[0050] S1. Obtaining a blood sample of a human or animal body after antigen immunization, and dividing the blood sample into a first sample and a second sample;

[0051] S2. Perform B cell receptor sequencing on the first sample to obtain a first sequencing result;

[0052] S3. Perform single-cell VDJ sequencing of B cells on the second sample to obtain a second sequencing result; the condition for the single-cell VDJ sequencing is to use a 10x Genomics Immune Profiling sequencing solution.

[0053] S4. Perform antigen-specific clustering on the first sequencing result and the second mRNA sequencing result to obtain B cell receptor heavy chain mRNA clusters; the screening conditions for the antigen-specific clustering include: (1) within the cluster Contains heavy chain sequences from single-cell VDJ sequencing; (2) The amino acid sequences of all heavy chain CD...

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Abstract

The present disclosure relates to a method for preparing an antibody, an antibody, a nucleic acid encoding the antibody, and an engineered cell. The antibody provided by the invention can resist severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and is good in specificity, high in sensitivity and excellent in pathogen neutralization effect.

Description

technical field [0001] The present disclosure relates to the field of biotechnology, in particular, to a method for preparing an antibody, an antibody, a nucleic acid encoding the antibody, and an engineered cell. Background technique [0002] Neutralizing antibodies are antibodies that are produced when pathogenic microorganisms invade the body. When pathogenic microorganisms invade cells, they need to rely on the specific molecules expressed by the pathogen itself to bind to the receptors on the cells to infect the cells and further expand. Neutralizing antibodies are certain antibodies produced by B lymphocytes, which can bind to antigens on the surface of pathogenic microorganisms, thereby preventing the pathogenic microorganisms from adhering to target cell receptors and invading cells. [0003] COVID-19, the novel coronavirus pneumonia caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has spread globally as a severe disease pandemic (Callaway et ...

Claims

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Application Information

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IPC IPC(8): C07K16/10C12N15/13C12N5/10
Inventor 蒋庆华王平平罗檬周文洋许召春靳喜云
Owner 蒋庆华
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