Method for preparing feed additive containing antibacterial peptide

A feed additive and antibacterial peptide technology, which is applied in the field of feed additives, can solve the problems of easy decomposition of antibacterial peptides, lack of digestion and absorption of antibacterial peptides, and no elimination of miscellaneous bacteria, so as to enhance physical fitness and immunity, and promote intestinal absorption of animals. The effect of increasing activity and content

Pending Publication Date: 2022-04-26
清远一生自然生物研究院有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current methods for preparing feed additives containing antimicrobial peptides still have many deficiencies in the operation process: on the one hand, the current feed additives have the problems of toxicity, poor sweetness, poor taste and low nutritional content in the preparation process, and in the preparation process In this method, the bacteria in the feed additives are not eliminated, which leads to the subsequent process of compounding with the antimicrobial peptides to be easily killed by the antimicrobial peptides, thereby making the antimicrobial peptides inactive; on the other hand, the existing antimicrobial peptides are being prepared Microbial fermentation is not carried out during the process, resulting in the antimicrobial peptides not having the effect of promoting digestion and absorption, and the antimicrobial peptides are easy to decompose during the preparation process

Method used

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  • Method for preparing feed additive containing antibacterial peptide

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Effect test

Embodiment 1

[0029] Embodiment 1, with reference to figure 1 , a method for preparing a feed additive containing antimicrobial peptides, comprising the following steps:

[0030] S1: Materials: Take 10-20 parts of dry yam powder, 5-10 parts of stevioside, 3-6 parts of sodium glutamate, 4-8 parts of plant essential oil, 1-4 parts of Bacillus, bifidus 2-5 parts of bacilli, 1-3 parts of honeysuckle, 6-12 parts of protein powder, 8-12 parts of vitamins, 3-5 parts of bentonite, 2-4 parts of yeast and 3-5 parts of honey ;

[0031] S2: Grinding and stirring: Put the solid raw materials taken from S1 into the pulverizer together and set the pulverizing time for sufficient pulverization. After the pulverization is completed, add the pulverized solid raw materials and the liquid raw materials together to the temperature-adjusting magnetic stirring The instrument carries out high-temperature stirring and mixing;

[0032] S3: Aerobic fermentation: Add the stirred and mixed solution in S2 into an aer...

Embodiment 2

[0038] Embodiment 2, with reference to figure 1 , the present embodiment is optimized on the basis of Example 1, a method for preparing a feed additive containing antimicrobial peptides, further comprising the following steps:

[0039] S4: Strain rejuvenation: Gram-negative strains are lyophilized at low temperature for a period of time, and then the lyophilized Gram-negative strains are inoculated on the medium for re-growth;

[0040] S5: Fermentation of strains: put the Gram-negative strains after complex growth in S4 into the medium for microbial fermentation treatment, and at the same time add an appropriate amount of probiotic microorganisms to the microbial fermentation tank, and ventilate and ferment in a ventilated environment;

[0041] S6: Fermentation broth pretreatment: filter and concentrate the mash after fermentation in S5, add stabilizer carrier to the filtrate after concentration, mix evenly, evaporate and crystallize to form a solid;

[0042] S7: Antimicrobia...

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Abstract

The invention belongs to the technical field of feed additives, particularly relates to a method for preparing a feed additive containing antibacterial peptides, and provides the following scheme aiming at the problems proposed in the background technology: the method comprises the following steps: S1, taking materials; s2, crushing and stirring; s3, aerobic fermentation; s4, rejuvenating the strains; s5, strain fermentation; s6, pretreatment of fermentation liquor; s7, antibacterial peptide extraction; and S8, compounding the antibacterial peptide with a feed additive. Probiotic microorganisms are added in the strain fermentation process in the step S5, and a stabilizer is added in the fermentation liquor pretreatment in the step S6 to stabilize the fermentation liquor, so that the fermentation liquor is prevented from being decomposed, the activity is improved, and the content is increased; and finally, the prepared feed additive is combined, so that the prepared feed additive containing the antibacterial peptide is high in nutrient content, beneficial to improving the growth rate of animals, beneficial to promoting intestinal absorption of the animals and beneficial to enhancing physique and immunity.

Description

technical field [0001] The invention relates to the technical field of feed additives, in particular to a method for preparing a feed additive containing antimicrobial peptides. Background technique [0002] In the process of modern livestock and poultry farming, in order to prevent and treat animal diseases, excessive antibiotic products are often used to ensure the health of animals, but the excessive use of antibiotics often leads to the decline in the quality of animal meat products and their products and the enhancement of antibiotic resistance, resulting in transmission through the food chain. Human health is also seriously affected by the problem of antibiotics. In order to reduce the problem of antibiotic residues in livestock and poultry farming, increase productivity, and reduce environmental pollution from the livestock and poultry industry, the development of feed additives has become a hot spot in recent years. Among them, feed additives based on antimicrobial p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/30A23K20/163A23K20/158A23K20/142A23K10/12A23K20/147A23K20/174A23K20/28A23K10/20A23K20/105A23K20/195A23K10/18C07K1/14C12P21/00
CPCA23K10/30A23K20/163A23K20/158A23K20/142A23K10/12A23K20/147A23K20/174A23K20/28A23K10/20A23K20/105A23K20/195A23K10/18C12P21/00C07K1/145A23V2400/51A23V2400/169
Inventor 林万华黄钦耿黄炜乾唐谢芳王珍珍吴森雨蒋顺进
Owner 清远一生自然生物研究院有限公司
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