Automatic membrane washing device for Western Blot experiment
An experimental and membrane washing technology, applied in cleaning methods and utensils, cleaning methods using liquids, chemical instruments and methods, etc., can solve problems such as long time, affecting the shaking of the membrane washing box, etc., to achieve the effect of saving labor
Active Publication Date: 2022-05-10
WEST CHINA HOSPITAL SICHUAN UNIV
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Problems solved by technology
This device can easily add and discharge the membrane washing solution, but it needs the pipeline to be connected to the membrane washing box all the time, and the pipeline has a certain drag on the membrane washing box, which affects the shaking of the membrane washing box
Furthermore, this device actually needs to manually switch the pipeline to add the membrane washing solution into the capsule, and also needs to manually switch the pipeline to discharge the membrane washing solution, which actually takes up a lot of time for the experimenter.
Method used
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Embodiment Construction
[0023] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.
[0024] Such as Figure 1 to Figure 3 As shown, the automatic film washing device for Western Blot experiments includes a horizontal shaker 1, and the horizontal shaker 1 includes a shaker host 11 and a shaker plate 12; it also includes a bellows 2, a lifting device 4, a pumping and discharging device 5 and a controller; The bellows 2 is fixed on the shaking plate 12. There is a vertical partition 21 inside the bellows 2. The vertical divider 21 divides the inner space of the bellows 2 into a membrane washing chamber 22 and a suction chamber 23. The bottom of the vertical divider 21 has a connecting wash The communication hole 211 of the membrane chamber 22 and the suction chamber 23; the lifting device 4 is connected with the shaker main machine 11; The film washing liquid is injected into the suction cavity 23 and the film washing liquid in t...
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The invention discloses an automatic membrane washing device for a Western Blot experiment, and provides the automatic membrane washing device for the Western Blot experiment, which can automatically realize membrane washing. The automatic membrane washing device for the Western Blot experiment comprises a horizontal shaking table, wherein the horizontal shaking table comprises a shaking table main machine and a shaking disc; the device further comprises a diaphragm capsule, a lifting device, a liquid pumping and draining device and a controller. The diaphragm capsule is fixed on the shaking disc, a vertical partition plate is arranged in the diaphragm capsule and divides the internal space of the diaphragm capsule into a diaphragm washing cavity and a suction cavity, and a communicating hole for communicating the diaphragm washing cavity with the suction cavity is formed in the bottom of the vertical partition plate; the lifting device is connected with the shaking table host; the liquid pumping and draining device is connected with the lifting device, and when the lifting device descends, the liquid pumping and draining device can inject film washing liquid into the film box and pump out the film washing liquid in the pumping cavity; and the controller controls the shaking table host, the lifting device and the liquid pumping and draining device to work.
Description
technical field [0001] The invention relates to the field of medical experiment devices, in particular to an automatic membrane washing device for Western Blot experiments. Background technique [0002] Western blotting is Western Blot. It is an experimental method commonly used in molecular biology, biochemistry and immunogenetics. The basic principle is to stain the cells or biological tissue samples treated by gel electrophoresis through specific antibodies. Information about the expression of specific proteins in the analyzed cells or tissues can be obtained by analyzing the location and depth of staining. [0003] There are many steps in Western Blot experiments, including primary antibody incubation, first membrane wash, secondary antibody incubation, and second membrane wash. Both the first membrane wash and the second membrane wash are carried out on a horizontal shaker. Specifically, the membrane cassette is placed on the shaker, the membrane wash solution is pou...
Claims
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IPC IPC(8): B08B3/10B08B13/00
CPCB08B3/044B08B13/00
Inventor 魏全黄礼义孙欣张庆程鸿馨
Owner WEST CHINA HOSPITAL SICHUAN UNIV