Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Primer, kit and method for functional marker detection of rice fragrance gene badh2

A technology for labeling detection and fragrance genes, which is applied in the fields of botanical equipment and methods, biochemical equipment and methods, and plant gene improvement, and can solve the problems of complex functional labeling operations and high costs.

Pending Publication Date: 2022-05-31
海南大学三亚南繁研究院
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the functional site of badh2 has been identified and some corresponding functional markers have been reported, there are still disadvantages such as complicated operation and high cost in the early development of functional markers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer, kit and method for functional marker detection of rice fragrance gene badh2
  • Primer, kit and method for functional marker detection of rice fragrance gene badh2
  • Primer, kit and method for functional marker detection of rice fragrance gene badh2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Development of functional markers for rice scent gene badh2

[0023] 1. Functional marker primer design and verification

[0024] According to the sequence characteristics of the rice non-scented gene Badh2 (nucleotide sequence as shown in SEQ ID No.1) and the scented gene badh2 gene (nucleotide sequence as shown in SEQ ID No.2) and the 8 base differences in the seventh exon region, the primer pairs of nucleotide sequences such as SEQ ID No. 3 (primer Xian7F) and SEQ ID No.4 (primer Xian7R) were designed for the expansion region containing the difference region of the seventh exon of the badh2 gene (see). Figure 1 In the figure, badh2 is the scent gene sequence, Bahd2 is the scented gene sequence, the numbers 142, 218 represent the number of uninsettled nucleotides, and the positions and sequences of each primer are marked on the figure), whether it is a band of about 440 bp can be amplified for fragrant rice, the amplified fragment can be used as an internal refe...

Embodiment 2

[0047] Example 2 is suitable for the application of rice flavor gene badh2 functional marker

[0048] Firstly, the total DNA of the rice parental material to be improved was extracted, and a PCR system composed of four primers shown in the nucleotide sequences such as SEQ ID No.3, SEQ ID No.4, SEQ ID No.7, and SEQ ID No.8 was used for testing, so as to determine whether each parental material carried the fragrance gene badh2:

[0049] (1) If the PCR fragments of 441 bp and 204 bp can be amplified in the sample to be tested, it indicates that the pure flavor gene badh2 is carried in the rice material to be improved;

[0050](2) If the PCR fragments of 441 bp and 288 bp can be amplified in the test material, it shows that the non-fragrance gene Badh2 is homozygous in the rice material to be improved;

[0051] (3) If the PCR fragments of 441 bp, 204 bp and 288 bp can be amplified in the test sample, it indicates that the test sample is a badh2 and badh2 heterozygous genotype.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a primer, a kit and a method for functional marker detection of a rice fragrance gene badh2. In a four-primer PCR amplification system, the nucleotide sequences of primer pairs are shown as SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.7 and SEQ ID NO.8. In the four-primer PCR amplification system, the nucleotide sequences of the primer pairs are shown as SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.7 and SEQ ID NO.8; the badh2 gene is a rice fragrance gene; the Badh2 gene is a rice non-fragrant gene. Aiming at the defects of complex operation and high cost of a detection marker of a mutation type of a seventh exon of a badh2 gene, the invention provides a development method of a functional marker of the aroma gene badh2 in order to more quickly and more effectively apply the aroma gene badh2 to a rice quality breeding project. The functional marker designed by the method can improve the accuracy of the badh2 gene in molecular marker-assisted selective breeding and reduce the detection cost.

Description

Technical field [0001] The present invention relates to a primer for detecting rice scent gene, specifically to a primer and a kit and method for functional marker detection of rice scent gene badh2. Background [0002] Fragrant rice is loved by many consumers because of its rich aroma and excellent taste. With the improvement of people's living standards, the demand for fragrant rice has increased. The cultivation of fragrant rice varieties uses traditional breeding methods with low efficiency, low accuracy and high cost, and molecular marker-assisted selection can solve various problems caused by traditional breeding. [0003] In view of the importance of aroma traits, the study of the cultivation and aroma production mechanism of fragrant rice varieties has been valued by many breeding experts. Predecessors have conducted a lot of research on the aromatic composition of fragrant rice, and most of the research results show that the main component of the aroma of fragrant rice i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11A01H1/02
CPCC12Q1/6895C12Q1/6858A01H1/02C12Q2600/13C12Q2600/16C12Q2600/156C12Q2531/113C12Q2537/143
Inventor 夏志辉任小青苗哲宁冀占东刘士饶杨小锋
Owner 海南大学三亚南繁研究院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com