Method for detecting taurine content in formula milk powder for special medical purpose

A technology of formula milk powder and detection method, which is applied in the direction of measuring devices, scientific instruments, instruments, etc., can solve the problems of incomplete taurine derivatization reaction, large relative standard deviation, inability to accurately quantify taurine content, etc., and achieve shortened column Effects of pre-derivation time, high accuracy, fast derivation speed and efficiency

Pending Publication Date: 2022-06-03
SHANGHAI INST OF QUALITY INSPECTION & TECHN RES
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Problems solved by technology

In the national standard GB5009.169-2016, the traditional infant formula milk powder is derivatized with 1.5 mg/mL DNS-CL derivative solution under alkaline conditions after protein precipitation to obtain fluorescent products. Although the above method is used to detect The recovery rate of the given taurine can reach more than 90%; but because in the total amino acid hydrolysis

Method used

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  • Method for detecting taurine content in formula milk powder for special medical purpose
  • Method for detecting taurine content in formula milk powder for special medical purpose
  • Method for detecting taurine content in formula milk powder for special medical purpose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Selection and optimization of detection methods

[0044] 1. Preparation of standard working solution

[0045] Accurately weigh 100.0 mg of taurine standard in a 100 mL volumetric flask, and prepare a standard stock solution with a concentration of 1.0 mg / mL using water as a solvent. Before the experiment, the standard stock solution was diluted with water into standard working solutions with concentrations of 1.0, 5.0, 10.0, 15.0, and 20.0 μg / mL.

[0046] 2. Extract taurine from the milk powder sample to be tested: accurately weigh 1.00±0.05g milk powder sample into a 50mL polytetrafluoroethylene plastic centrifuge tube, add about 35mL ultrapure water, ultrasonicate for 15min, and add 1mL 150g / L ferrocyanide The potassium chloride solution and 1 mL of 300 g / L zinc acetate solution were vortexed for 5 min to uniformly mix to remove the protein in the matrix, centrifuged at 9000 r / min for 2 min, and the supernatant was transferred to a 50 mL volumetric flask, a...

Embodiment 2

[0061] Example 2 Influence of different DNS-CL derivative liquid concentrations

[0062] In this example, the whole amino acid hydrolyzed formula milk powder is used as the sample to be tested, and the theoretical addition amount of taurine in the sample is 40.0 mg / 100 g. The sample was extracted and derivatized using the method in Example 1, and the prepared sample-derived product was detected by a two-dimensional liquid phase system. In this example, by changing the concentration of the derivatization reagent DNS-CL derivative solution, the effect of the concentration of the derivatization reagent on the detection of the taurine content in the milk powder samples for special medical care was investigated. The sample processing and detection methods are the same as in Example 1, except that the concentrations of the DNS-CL derivative solutions of different groups are set. Considering that the solubility of DNS-CL in acetonitrile is 50 mg / mL, the concentrations of the DNS-CL d...

Embodiment 3

[0066] Example 3 The influence of the pH value of the derived environment

[0067] During the reaction of taurine with DNS-CL, with the fluorescent product dansyl-aminoethanesulfonic acid DNS-NH-(CH 2 ) 2 –SO 3 Generation of H, Cl on DNS-CL - with H + Combined with HCl, it reduces the pH of the solution and inhibits the reaction in the positive direction. Therefore, during the derivatization process, it is necessary to keep the solution system in an alkaline environment. In this experiment, the whole amino acid hydrolyzed formula milk powder was also used as the sample to be tested, and the theoretical addition amount of taurine in the milk powder sample was 40.0 mg / 100 g. The method in Example 1 was used to extract and derivatize the sample, and the product derived from the sample was prepared and detected by a two-dimensional liquid phase system. The sample processing and detection methods are the same as those in Example 1, the difference is that the pH of the derivati...

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Abstract

The invention provides a method for detecting the content of taurine in formula milk powder for special medical purposes. The method comprises the following steps: (1) preparing a taurine standard substance working solution; (2) extracting taurine: accurately weighing a to-be-detected milk powder sample, dissolving the to-be-detected milk powder sample in water, performing ultrasonic treatment, adding a precipitator, uniformly mixing, centrifugally extracting supernate, and fixing the volume to obtain a to-be-detected sample solution; (3) derivatization treatment: respectively adding a derivatization reagent dansyl chloride into the standard substance working solution and the to-be-detected sample solution prepared in the steps (1) and (2), and carrying out a derivatization reaction in an alkaline environment to obtain a standard substance derivative product and a sample derivative product; and (4) detection and analysis: detecting the content of the taurine derivative product in the sample derivative liquid prepared in the step (3) by adopting a two-dimensional liquid phase system. According to the method, a sample pretreatment method is designed to be combined with a two-dimensional liquid phase system, so that the problem that the content of taurine in the special medical milk powder cannot be accurately analyzed in the national standard is solved, and the detection accuracy is high.

Description

technical field [0001] The invention relates to the technical field of milk powder detection, in particular to a detection method for taurine content in formula milk powder for special medical purposes. Background technique [0002] Taurine (Taurine, C 2 H 7 NO 3 S,HO 3 S-CH 2 -CH 2 -NH 2 ) The chemical name is β-aminoethanesulfonic acid, which is a special sulfur-containing non-protein amino acid, which is distributed in the interstitial fluid and intracellular fluid in a free state. Taurine has outstanding physiological and pharmacological effects, can regulate the metabolism of carbohydrates and lipids in the body, improve immunity, maintain osmotic pressure balance, enhance the antioxidant capacity of cell membranes, etc. Development also plays an important role. At present, taurine has been added to food as a necessary nutritional fortifier. Developed countries such as Europe and the United States stipulate that a certain amount of taurine must be added to infan...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/74G01N30/8679G01N2030/067
Inventor 卞华翁史昱王毕悦
Owner SHANGHAI INST OF QUALITY INSPECTION & TECHN RES
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