Preparation method of novel crown IgG/IgM antibody detection test strip

Abstract

The invention relates to the field of biological analysis and medical treatment, and discloses a preparation method of a novel crown IgG / IgM antibody detection test strip. A combination pad is prepared by using nanogold marked by a certain proportion of mixed recombinant new coronavirus N protein and chicken IgY, and mouse anti-human IgM and mouse anti-human IgG diluted by antibody coating liquid and rabbit anti-chicken IgY diluted by antibody coating liquid are respectively used as a T line and a C line. The test strip is prepared by sequentially assembling the sample pad, the combination pad, the NC membrane and the absorbent paper. The preparation method provided by the invention can provide a treatment method of the sample pad and a pretreatment and preparation method of the combination pad for preparing the novel coronavirus antibody colloidal gold test strip; the preparation method of the antibody-coated nitrocellulose membrane has important significance in the fields of biological analysis and medical treatment.

Description

technical field [0001] The invention relates to a preparation method of a novel coronavirus IgG / IgM antibody detection test strip, which is designed to be used in the fields of biological analysis and medical treatment. Background technique [0002] Due to the high requirements of nucleic acid detection kits on the testing environment and testing personnel, as well as the popularity of specialized laboratories for nucleic acid testing, the basic level and large-scale screening, promotion and application of nucleic acid testing kits have been greatly limited. At present, in addition to nucleic acid detection methods, rapid screening is also feasible by detecting the protein (antigen) of the virus itself or the antibodies produced in the body after virus infection. The present invention aims at 2019-nCoV N protein-specific antibodies, combined with colloidal gold immunochromatography technology, develops colloidal gold chromatography detection and detection that can capture se...

AI Technical Summary

Problems solved by technology

[0002] Due to the high requirements of nucleic acid detection kits on the testing environment and testing personnel, as well as the popularity of specialized laboratories for nucleic acid testing, this greatly limits the basic level and large-scale screening, promotion and application of nucleic acid testing kits at home.