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Cell separation equipment and application of cell separation equipment to treatment of leukemia by utilizing vertical reduction, progressive increase and acceleration

A technology for separating equipment and cells, which is applied in the field of cell separation to achieve the effect of facilitating extraction and improving removal efficiency

Pending Publication Date: 2022-07-05
罗自勉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the treatment of leukemia, it is necessary to extract the lymphocytes in the patient's blood for analysis. At present, the blood lymphocytes are extracted by centrifugation. By adding the blood sample PBS mixture into the centrifuge tube with the lymphocyte separation medium, the blood sample is located in the extraction solution as much as possible. In the upper layer, do not use too much force to spread the blood cells to the lower layer, otherwise there is no way to centrifuge and stratify, and then use the centrifuge tube to perform centrifugal motion to extract lymphocytes

Method used

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  • Cell separation equipment and application of cell separation equipment to treatment of leukemia by utilizing vertical reduction, progressive increase and acceleration
  • Cell separation equipment and application of cell separation equipment to treatment of leukemia by utilizing vertical reduction, progressive increase and acceleration
  • Cell separation equipment and application of cell separation equipment to treatment of leukemia by utilizing vertical reduction, progressive increase and acceleration

Examples

Experimental program
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Effect test

Embodiment 1

[0031] see Figure 1-2, a cell separation device, comprising a first support frame 1, a first motor 2 is installed at the bottom of the first support frame 1, and the output end of the first motor 2 is connected with a liquid mixing bucket 4 through a first shaft 3, which is located at a convenient location for observation and separation. In some cases, the mixing bucket 4 is made of transparent material, the first rotating shaft 3 penetrates the first support frame 1, the first rotating shaft 3 is rotatably connected with the first support frame 1, the surface of the mixing bucket 4 is fixedly connected with a feeding pipe 5, and the first The surface of the support frame 1 is fixedly connected with a second support frame 6, the surface of the second support frame 6 is mounted with a second motor 7, and the output end of the second motor 7 is fixedly connected with a screw rod 8, and the screw rod 8 is connected to the second support frame. 6. Rotationally connected, the oute...

Embodiment 2

[0034] This embodiment is an improvement made on the basis of Embodiment 1. On the basis of the accompanying drawings of Embodiment 1, please combine the attached drawings again. Figure 3-4 .

[0035] A third motor 16 is mounted on the surface of the second support frame 6 , the output end of the third motor 16 is connected with a first gear 18 through the second rotating shaft 17 , the side wall of the first gear 18 is engaged with a second gear 19 , and the second gear 19 is engaged with the second gear 19 . The inner wall of the gear 19 is coaxially provided with a second liquid inlet pipe 20, one end of the second liquid inlet pipe 20 is rotatably connected with the first liquid inlet pipe 12, and the other end of the second liquid inlet pipe 20 is fixedly connected with the scraper 13. The outer wall of the second liquid inlet pipe 20 is symmetrically and fixedly connected with sliding bars 22 , and the inner wall of the second gear 19 is symmetrically opened with first ...

Embodiment 3

[0039] This embodiment is an improvement made on the basis of Embodiment 2. On the basis of the attached drawings of Embodiment 2, please combine the attached drawings again. Figure 5-6 .

[0040] The side wall of the spatula 13 is fixedly connected with a third support frame 25 , the inner wall of the third support frame 25 is rotatably connected with a first rotating rod 26 , and the outer wall of the first rotating rod 26 is fixedly connected with a first movable plate 28 . A first spring 27 is arranged between the plate 28 and the spatula 13 , and a limit bar 29 is arranged on the inner wall of the third support frame 25 , and the limit bar 29 is used to limit the rotation angle of the first movable plate 28 .

[0041] The outer wall of the first rotating rod 26 is fixedly connected with the third gear 30, the side wall of the third gear 30 is meshed and connected with the fourth gear 31, and the fourth gear 31 is rotatably connected with the third supporting frame 25 thr...

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Abstract

The invention discloses cell separation equipment and application to treatment of leukemia by means of vertical decreasing and progressive increasing acceleration, and relates to the technical field of cell separation.The cell separation equipment comprises a first supporting frame, a second supporting frame and a first motor, the output end of the first motor is connected with a liquid mixing barrel through a first rotating shaft, and the surface of the liquid mixing barrel is fixedly connected with a feeding pipe; a second motor is installed on the surface of the second supporting frame, the output end of the second motor is fixedly connected with a lead screw, the outer wall of the lead screw is in threaded connection with a moving plate, guide rods are symmetrically and fixedly connected to the inner wall of the second supporting frame, the moving plate is in sliding connection with the guide rods, and a suction pump is installed on the surface of the moving plate; a liquid inlet of the suction pump is connected with a spatula through a first liquid inlet pipe, a liquid outlet of the suction pump is connected with a collecting barrel through a liquid outlet pipe, and the collecting barrel is installed on the surface of the first supporting frame. And the extraction of lymphocytes is facilitated.

Description

technical field [0001] The present invention relates to the technical field of cell separation, and more particularly, to a cell separation device and the application of using instant reduction, increasing speed and increasing speed to treat leukemia. Background technique [0002] Lymphocytes are a type of leukocytes and the smallest leukocytes. They are produced by lymphoid organs and mainly exist in the lymph fluid circulating in the lymphatic vessels. They are an important cellular component of the body's immune response function and are responsible for almost all immune functions of the lymphatic system. The main executor is the front-line "soldier" who fights external infections and monitors cell mutation in the body. [0003] During leukemia treatment, it is necessary to extract lymphocytes in the blood of patients for analysis. At present, blood lymphocytes are extracted by centrifugation. By adding the blood sample PBS mixture into a centrifuge tube with lymphocyte s...

Claims

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Application Information

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IPC IPC(8): B04B1/00B04B11/08B04B11/06
CPCB04B1/00B04B11/082B04B11/06
Inventor 罗自勉杨霖刘平刘康谭振清朱开办
Owner 罗自勉
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