Fermentation method for high-yield production of docosahexaenoic acid by using schizochytrium limacinum and application of fermentation method

A technology of Schizochytrium and fermentation method, which is applied in the field of high-yield docosahexaenoic acid fermentation of Schizochytrium, can solve problems such as environmental pollution, inability to synthesize DHA by itself, and different product quality, so as to shorten fermentation time, The effect of improving production efficiency and increasing fermentation yield

Pending Publication Date: 2022-07-05
SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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Problems solved by technology

However, due to the lack of fatty acid dehydrogenase above △-9 in the human body, DHA cannot be synthesized by itself, so it can only be ingested externally.
At present, DHA products are mainly derived from deep-sea fish oil, but the product quality will vary with fish species, fishing seasons, and locations, and is susceptible to environmental pollution

Method used

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  • Fermentation method for high-yield production of docosahexaenoic acid by using schizochytrium limacinum and application of fermentation method
  • Fermentation method for high-yield production of docosahexaenoic acid by using schizochytrium limacinum and application of fermentation method
  • Fermentation method for high-yield production of docosahexaenoic acid by using schizochytrium limacinum and application of fermentation method

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Embodiment

[0044] 1 Materials and methods

[0045] 1.1 Strains

[0046] Schizochytrium limacinum SR21 (ATCC MYA1381) was purchased from the American Type Culture Collection.

[0047] Schizochytrium limacinum SFD-1502, a DHA-producing strain obtained by screening in the inventor's laboratory. The strain can be obtained by the public from Shandong Food Fermentation Industry Research and Design Institute.

[0048] 1.2 Culture medium

[0049] Artificial seawater formula: sodium chloride 11.2g / L, potassium chloride 0.8g / L, magnesium sulfate 1.9g / L, calcium sulfate 0.9g / L, magnesium chloride 2.6g / L.

[0050] Seed medium: glucose 50g / L, yeast powder 20g / L, sodium glutamate 5g / L, 50% v / v artificial seawater.

[0051] Glucose fermentation medium: glucose 120g / L, yeast powder 11.75g / L, sodium glutamate 6.66g / L, ammonium sulfate 1g / L, 50% v / v artificial seawater.

[0052] Glycerol fermentation medium: glycerol 120g / L, yeast powder 11.75g / L, sodium glutamate 6.66g / L, ammonium sulfate 1g / L, 50% ...

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Abstract

The invention belongs to the technical field of microbial fermentation, and particularly relates to a high-yield docosahexaenoic acid fermentation method of schizochytrium limacinum and application of the high-yield docosahexaenoic acid fermentation method. The invention systematically studies the influence of the fermentation of two carbon sources of glucose and glycerol on the production of DHA by schizochytrium limacinum. Research finds that glucose and glycerol are mixed in proportion to serve as fermentation raw materials, consumption of two carbon sources can be mutually inhibited, and the production efficiency is lower than that of pure glycerol raw materials; if the thalli are cultured by using glucose firstly and glycerol is supplemented after the glucose is used up, the production efficiency is lower than that of a pure glycerol raw material. And furthermore, the problem of mutual inhibition of the two carbon sources of glucose and glycerol is solved by controlling the glycerol supplementing time, the respective advantages of the double carbon sources are fully played, and the production efficiency of producing DHA by the fission vibrio is improved, so that the cost is reduced, and the method has good practical production and application values.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, in particular to a high-yield docosahexaenoic acid fermentation method of Schizochytrium and application thereof. Background technique [0002] The disclosure of information in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] Docosahexaenoic acid (DHA) is a non-toxic and safe omega-3 polyunsaturated fatty acid, commonly known as brain gold, which can promote human visual organs, brain development and treat heart disease, Inflammation, cancer, anxiety and other aspects have unique physiological functions, which are especially important for the health of infants and young children in the growth and development stage. However, due to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/6434C12N1/38C12R1/645
CPCC12P7/6427C12N1/38
Inventor 赵祥颖张家祥张华秋刘丽萍姚明静赵晨刘建军
Owner SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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