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Polypeptide PFAP1 for activating primordial follicles so as to treat or assist in treating premature ovarian failure and application of polypeptide PFAP1

A technology for adjuvant treatment and premature ovarian failure, applied in the field of polypeptide PFPA1 and its application, to achieve the effect of strong specificity, easy absorption, and recovery

Pending Publication Date: 2022-07-22
NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there has not been any report on exploring a new method for activating primordial follicles from the perspective of endogenous polypeptides. Therefore, this topic intends to explore the effect and mechanism of endogenous polypeptides on the activation of primordial follicles

Method used

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  • Polypeptide PFAP1 for activating primordial follicles so as to treat or assist in treating premature ovarian failure and application of polypeptide PFAP1
  • Polypeptide PFAP1 for activating primordial follicles so as to treat or assist in treating premature ovarian failure and application of polypeptide PFAP1
  • Polypeptide PFAP1 for activating primordial follicles so as to treat or assist in treating premature ovarian failure and application of polypeptide PFAP1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Characteristics of polypeptides identified by quantitative LC / MS

[0049] 1.1 Sample Collection

[0050] We selected 4 patients who underwent gynecological inpatient surgery in the Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University (Nanjing Maternal and Child Health Hospital) from 2018 to 2019. Among them, 2 patients underwent ovarian biopsy due to cervical cancer and the pathology showed normal 2 different parts. The ovarian cortical tissue of 2 cases and 2 cases of POF complicated with cervical cancer underwent ovarian tissue excision and biopsy of normal ovarian cortical tissue in 2 different parts. All the selected patients were free of hypertension, diabetes, immune system and other diseases. The tissue collected during the operation was quickly transported to liquid nitrogen for storage. This study was approved by the Ethics Committee of the Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, and all pa...

Embodiment 2

[0055] Example 2: Assessing the role of differential polypeptides in the activation of primordial follicles in neonatal mice

[0056] 2.1 Mouse ovary culture and follicle count

[0057] The in vitro culture medium of ovarian tissue is DMEM / F12 medium containing 1% pen / streptomycin, 1% ITS, 0.05mg / ml vitamin C and 3mg / ml BSA, filtered with a 0.22 μm disposable filter and stored at 4°C spare. After the mouse ovaries were collected under a dissecting microscope, the mouse ovaries were transferred to a petri dish according to the instructions of the petri dish (Millicell, PICM01250), and cultured at 37°C, 5% CO 2 in a conventional incubator for 24 hours. The treated ovarian tissues of each group were fixed in 4% paraformaldehyde (Servicebio, China), dehydrated with various concentrations of alcohol on the next day, embedded in paraffin (Leica, Germany), and continuously performed at a thickness of 5 μm / sheet. slice. Sections were stained with hematoxylin (Solarbio, China). Wh...

Embodiment 3

[0061] Example 3: PFAP1 is an evolutionarily highly conserved lipophilic peptide, and can significantly promote the activation of primordial follicles;

[0062] 3.1 Analysis of the basic characteristics of the polypeptide PFAP1 and its precursor proteins

[0063] Preliminary bioinformatics analysis of PFAP1 was carried out, and the results showed that: ① The polypeptide precursor protein Histone H4 consists of 103 amino acids, and PFAP1 is located in the helical domain of amino acids 48-61 ( image 3 A); ② Using the Protpram website to analyze the basic characteristics of the peptide, the molecular weight of PFAP1 is 1563.81, the instability index is 8.99, and the lipid solubility index is 125.00. It is a stable and lipophilic small molecule polypeptide, which may easily enter cells through the cell membrane; ③The homology analysis of the peptide found that the peptide is highly conserved in human, mouse, rat, pig, bovine and other species ( image 3 B). FITC-labeled PFAP1 w...

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Abstract

The invention discloses a polypeptide PFAP1 for activating primordial follicles so as to treat or assist in treating premature ovarian failure and application of the polypeptide PFAP1. The invention discloses a polypeptide PFPA1 for activating primordial follicles so as to treat or assist in treating premature ovarian failure. The amino acid sequence of the polypeptide PFPA1 is SGLIYEETRGVLKV. The applicant screens the polypeptide PFAP1 with high expression abundance in normal ovarian cortex tissues and obviously reduced abundance in the ovarian cortex tissues of POF patients by virtue of a tandem mass spectrometry method, and the polypeptide PFAP1 can obviously promote the activation of primordial follicles. The PFAP1 has the characteristics of being easy to absorb, strong in specificity, not easy to accumulate in vivo, good in targeting property and the like, so that the PFAP1 possibly becomes a potential therapeutic drug for treating the POF.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a polypeptide PFPA1 for activating primordial follicles to treat or assist in the treatment of premature ovarian failure and applications thereof. Background technique [0002] The ovary is a unique and important organ in a woman and plays an important role in a woman's life. The reserve number of primordial follicles and the degree of atresia are precisely regulated in the process of female development, thereby ensuring the normal reproductive ability and reproductive life of women. However, with the changes in social economy, culture, living environment, work rhythm, etc., the incidence of ovarian diseases such as premature ovarian failure (POF) is increasing year by year, and it is accompanied by a younger age trend, which seriously affects women's physical and mental health and health. Quality of Life [1,2] . The current clinical treatment for POF is mainly hormone replacement the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P15/08
CPCC07K7/08A61P15/08A61K38/00
Inventor 徐娟苏亦平贾雪梅张妍王瀚彬侯晓静周晓美
Owner NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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