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Organ chip, integrated module and system thereof, preparation method and application

An organ-on-a-chip and integrated system technology, applied in chemical instruments and methods, biochemical equipment and methods, and components of television systems, etc., can solve the problems of decreased manufacturing consistency, difficult-to-manufacture arrayed organ chips, synchronous culture and perfusion, and cost increase and other issues, to achieve the effect of improving consistency and stability, promoting electrical signal transmission, and promoting orderly arrangement

Pending Publication Date: 2022-08-02
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the photolithography process is complicated, limited by the size of the wafer, it is difficult to manufacture arrayed organ chips for simultaneous culture and perfusion, and it is difficult to realize the integrated manufacturing of scaffolds and microfluidic chips, which brings about cost increases and manufacturing costs. decline in consistency

Method used

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  • Organ chip, integrated module and system thereof, preparation method and application
  • Organ chip, integrated module and system thereof, preparation method and application
  • Organ chip, integrated module and system thereof, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] like figure 1 , In this embodiment, an organ chip includes a bottom cover 1, PDMS films 2 and 3, and a top cover 4. The bottom cover 1 and the top cover 4 are provided with cavities, and the PDMS films 2 and 3 are arranged in an orderly manner. The fiber scaffold 21; the bottom cover 1, the PDMS films 2 and 3, and the top cover 4 are stacked in sequence, wherein the cavities of the bottom cover 1 and the top cover 4 are enclosed to form a cell culture chamber, and the ordered fiber scaffold 21 is located in the In the cell culture chamber, an electrical excitation element is also integrated in the top cover 4 .

[0055] The electrical excitation elements are two platinum wires 41 electrodes, the platinum wires 41 are inserted into the cell culture chamber and an electrical stimulator is externally connected.

[0056] like figure 2 , the PDMS film in this embodiment is two layers, the thickness of the single layer is 300 μm, and the ordered fiber scaffolds 21 of each ...

Embodiment 2

[0068] An organ chip array integration module, comprising a substrate 52 on which a multi-channel fluid perfusion device 53, a multi-channel electrical stimulation integrator 55 and the organ chip of Embodiment 1 are arranged, the organ chip array Arrange.

[0069] like Figure 4 , the organ chip is integrated in the microfluidic guide plate 56 , and the multi-channel fluid perfusion device 53 is connected to the microfluidic guide plate 56 through the inlet 54 of the culture medium capillary 57 . The multi-channel electrical stimulation integrator 55 includes a plurality of electrical stimulation connection holes 59, which are used to connect to the power supply and are provided with a number of wires, which are respectively connected to the platinum wires 41 of each organ chip. . The organ chip is fixed on the substrate by the positioning fixture 58 . The outer periphery of the base plate is also provided with a rubber support frame 51 .

[0070] The electrical excitatio...

Embodiment 3

[0072] like Figure 5 , an integrated system for automatic perfusion of organ chips, including a controller, an air pressure source 61, a liquid storage tube, an arrayed integrated module of organ chips and a waste liquid pool; the air pressure source 61 is connected to the liquid storage tube for providing liquid outlet power , the liquid storage tube is connected to the organ chip arrayed integrated module, the organ chip arrayed integrated module is an organ chip arrayed integrated module of Embodiment 2, and an external electrical stimulator 69 is connected. The waste pool is used to collect cell culture waste. The controller is provided with a host computer control interface 68, which is convenient for manipulation.

[0073] A pressure regulator 62 is provided between the air pressure source 61 and the liquid storage pipe.

[0074] The liquid storage tube includes a cell culture liquid storage tube 65 and a cell suspension liquid storage tube 63, and a switching valve a...

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PUM

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Abstract

The invention discloses an organ chip, an integrated module and system thereof, a preparation method and application. The organ chip comprises a bottom cover, a PDMS thin film and a top cover, cavities are formed in the bottom cover and the top cover, and an ordered fiber support is arranged in the PDMS thin film; the bottom cover, the PDMS thin film and the top cover are sequentially arranged in a stacked mode, a cell culture chamber is defined by cavities of the bottom cover and the top cover, the ordered fiber support is located in the cell culture chamber, and an electric excitation element is further integrated in the top cover. A module integrating a multi-channel electrical stimulator and an automatic perfusion system is connected with an arrayed organ chip, synchronous electrical stimulation and continuous fluid stimulation are applied to on-chip tissues, and the organ chip can be used for cell culture of high-throughput drug detection.

Description

technical field [0001] The invention belongs to the technical field of organ chip design and production, and particularly relates to an organ chip and its integrated module and system, preparation method and application. Background technique [0002] Similar to cardiomyocytes, nerve cells, skeletal muscle cells, etc., they are in a three-dimensional extracellular matrix (ECM) microenvironment for a long time in vivo. This binding force acts as an external mechanical signal to the intracellular space, directing the myofibrils to promote sarcomere alignment and inducing the formation of an ordered organization. On the other hand, such cells with electrophysiological properties transmit electrical signals through Purkinje fibers to achieve synchronized contraction and relaxation functions. When cultured in vitro, cells often fail to obtain a fully mature phenotype because they cannot simulate the three-dimensional environment and electrical signal transmission network in vivo....

Claims

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Application Information

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IPC IPC(8): B01L3/00C12M1/42C12M1/34C12M1/36C12M1/00C12M3/00C12Q1/02B81B7/02
CPCB01L3/502707B01L3/50273B01L3/502738C12M23/16C12M25/14C12M29/10C12M35/02C12M41/48C12M41/44C12M41/40C12M21/08G01N33/5008B81B7/02G01N2500/10B01L2400/0487B01L2400/0622B81B2201/05
Inventor 孙道恒刘玲玲许丰程健邱彬金航陈松月何功汉
Owner XIAMEN UNIV
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