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Human oocyte in-vitro maturation culture solution containing mesenchymal stem cell cytoplasm extract and culture method

A technology for in vitro maturation and culture of human oocytes, applied in the field of reproductive medicine, can solve problems that have not been reported, and achieve the effects of increasing the maturity rate, increasing the maturity of cytoplasm and nucleus, improving the quality and developmental potential

Pending Publication Date: 2022-08-02
SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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Problems solved by technology

[0005] However, there is no report about a human oocyte in vitro maturation medium and culture method containing mesenchymal stem cell cytoplasmic extract

Method used

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Embodiment

[0018] 1. Experimental materials and experimental methods

[0019] Patients who underwent IVF assisted pregnancy due to fallopian tube factors or male factors in the Reproductive Medicine Center of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine were divided into control group (culture system without mesenchymal stem cell cytoplasmic extract) and experimental group (culture system without mesenchymal stem cell cytoplasmic extract). The system contains mesenchymal stem cell cytoplasmic extract), and the conventional ovulation induction program is used to collect discarded immature oocytes (immature oocytes with a giant nucleolus in the cytoplasm or no polar body discharge in the perioval space, This is the immature GV stage or MI stage oocytes). These eggs are routinely discarded due to their immaturity and low developmental potential, and it is feasible to select them for research. The patients were used for this study after consent and sig...

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Abstract

The invention relates to the technical field of reproductive medicine, in particular to a human oocyte in-vitro maturation culture solution containing a mesenchymal stem cell cytoplasm extract, and the culture solution is prepared by adding sex hormone, antibiotics and the mesenchymal stem cell cytoplasm extract on the basis of an oocyte basic culture solution. The invention also provides a human oocyte in-vitro maturation culture method. The invention provides a more practical and effective measure for improving an immature oocyte in-vitro maturation culture system. The culture solution disclosed by the invention can be used for remarkably improving the maturation rate, the fertilization rate, the cleavage rate and the blastocyst formation rate of immature oocytes in a foaming period.

Description

technical field [0001] The invention relates to the technical field of reproductive medicine, in particular to a human oocyte in vitro maturation culture medium and a culture method containing mesenchymal stem cell cytoplasmic extract. Background technique [0002] In vitro maturation (IVM) technology of immature oocytes is a kind of assisted reproductive technology. It is a mature oocyte in the second meiotic stage (Metaphase II, MII stage), which has the ability to fertilize and develop into a normal embryo. With the application and development of IVM technology in the field of assisted reproductive technology, infertile women of childbearing age, especially those with stubborn follicular maturation disorders, can get pregnant. [0003] The current commercialized human immature oocyte in vitro maturation medium, although it simulates the in vivo maturation environment of human oocytes, contains HCG, gonadotropin, estradiol, human serum albumin, etc., but has a low pregnan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/075C12N5/0775
CPCC12N5/0609C12N5/0662C12N2501/31C12N2500/84C12N2509/10
Inventor 童国庆尹萍孙宁宇张志航张武文李凯冯煜
Owner SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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