Method for determining faeces brain thioglycoside based on flight mass spectrometry

A technology of flight mass spectrometry and sulfatide, which is applied in the field of determination of fecal sulfatide based on flight mass spectrometry, can solve the problems of poor binding, poor controllability, and insufficient adaptability, and achieve strong controllability , Enhanced operational safety and adaptability, and high promotional value

Inactive Publication Date: 2022-08-05
河北省人民医院
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the combination of flight mass spectrometry and sulfatide is poor in actual operation, and the controllability is not good, and blood samples are usually taken during sam...

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  • Method for determining faeces brain thioglycoside based on flight mass spectrometry

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Embodiment Construction

[0027] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0028] see figure 1 Shown: the present invention is a method for measuring fecal cerebrosulfatide based on flight mass spectrometry, comprising the following steps:

[0029] Step S1: take a small amount of feces (about 1 gram) and add Hexane (n-hexane): isopropanol (isopropanol) (3:2) in a mixed solution to separate and extract sulfatide;

[0030] Step S2: use a centrifugal evaporation dryer to evaporate the purified sulfatide, add 3...

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Abstract

The invention discloses a method for determining faeces brain thioglycoside based on flight mass spectrometry, and relates to the technical field of mass spectrometry detection. The method comprises the following steps: S1, taking 1g of excrement, adding the excrement into a mixed solution composed of normal hexane and isopropanol, and separating and extracting thiocerebroside; s2, evaporating the purified sulfur cerebroside to dryness, adding 300 microliters of 0.1 N sodium hydroxide, and saponifying in a constant temperature box for 10 minutes to obtain hydrogenated sulfur cerebroside; s3, eluting other lipids by using a high-precision single-head filtering device, and purifying hydrogenated sulfur cerebroside; s4, dissolving the hydrogenated sulfur cerebroside purified in the high-precision single-head filtering device by using methanol, and adding a fixed amount of hydroxylated sulfur cerebroside; s5, completing the analysis of the lipid and the quantity of the hydrogenated sulfur cerebroside by using a matrix-assisted laser desorption ionization time-of-flight mass spectrometer; and S6, completing. According to the method disclosed by the invention, non-invasive determination can be realized in a mode of changing sampling into blood collection into excrement for determination, and the safety and the adaptability are enhanced.

Description

technical field [0001] The invention relates to the technical field of mass spectrometry detection, in particular to a method for determining fecal cerebrosulfatide based on flight mass spectrometry. Background technique [0002] Sulfatide is a kind of glycolipid that exists in large quantities in the human body. It is an important component of the nervous system. Many research results show that sulfatide has important physiological functions in the tissues and organs where it is located. The development of cerebroside detection methods and the progress of sulfatide-related research have found that sulfatide not only plays a unique role in the nervous system, but also has an irreplaceable role in the pathogenesis of pathophysiology in other systems. The full name of mass spectrometry in flight is surface-enhanced laser desorption ionization time-of-flight mass spectrometry technology. In the detection system of mass spectrometry in flight, the signal is converted and recorde...

Claims

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Application Information

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IPC IPC(8): G01N27/64
CPCG01N27/64
Inventor 李刚
Owner 河北省人民医院
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