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Lactobacillus curvatus capable of promoting generation of short-chain fatty acid in intestinal tract and application of lactobacillus curvatus

A Lactobacillus campylobacter, product technology, applied in the fields of medicine and microbiology, to achieve good safety, reduce colon shortening, and reduce the effect of disease activity index

Pending Publication Date: 2022-08-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at this stage, no researchers have paid attention to the effect of Lactobacillus flexus on the intestinal short-chain fatty acid content of colitis mice

Method used

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  • Lactobacillus curvatus capable of promoting generation of short-chain fatty acid in intestinal tract and application of lactobacillus curvatus
  • Lactobacillus curvatus capable of promoting generation of short-chain fatty acid in intestinal tract and application of lactobacillus curvatus
  • Lactobacillus curvatus capable of promoting generation of short-chain fatty acid in intestinal tract and application of lactobacillus curvatus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Example 1: Screening, identification and culture of Lactobacillus Campylobacter CCFM1268

[0087] 1. Filter

[0088] The fermented kimchi water from Sichuan Province was used as the sample. After the sample was pretreated, it was stored in a refrigerator at -80 °C in about 30% glycerol. After taking out and thawing, the sample was mixed and 0.2 mL of the sample was added to 5 mL of LBS medium. After enrichment for 8 hours, the enriched solution was diluted with 0.9% normal saline, and the appropriate gradient dilution was coated on LBS solid medium, cultured at 37°C for 48 hours, and typical colonies were picked and streaked on the MRS plate for purification. A single colony was picked and transferred to liquid MRS liquid medium for enrichment, and stored in 30% glycerol to obtain strain CCFM1268.

[0089] 2. Identification

[0090] Extract the genome of the screened Lactobacillus campylobacter, amplify and sequence its 16S rDNA (completed by Shanghai Sangon Bioengine...

Embodiment 2

[0094] Example 2: Effects of Lactobacillus Campylobacter CCFM1268 on colon length, body weight change, disease activity index and colon tissue in mice with colitis

[0095] Thirty-two healthy female C57 mice aged 6-8 weeks were randomly divided into 4 groups, with 8 mice in each group. The 4 groups were: blank control group, modeling group, and intragastric administration of Lactobacillus campylobacter CCFM1268 and Lactobacillus sake QJSNT1L10 respectively. Bacterial suspension CCFM1268, QJSNT1L10 intervention group. Among them, each mouse in the blank group and the model group was intragastrically administered with normal saline at a dose of 0.2 mL once a day, and each mouse in the other groups was intragastrically administered 1×10 at a dose of 0.2 mL 9 CFU / mL bacterial suspension. During the experiment, the blank group was given normal drinking water, and the other groups were given 25g / L DSS drinking water. After the experiment, each mouse was anesthetized with isofluran...

Embodiment 3

[0107] Example 3: The effect of Lactobacillus campylobacter CCFM1268 on the expression of tight junction protein in the colon of mice with colitis

[0108] Thirty-two healthy female C57 mice aged 6-8 weeks were randomly divided into 4 groups, with 8 mice in each group. The 4 groups were: blank control group, modeling group, and intragastric administration of Lactobacillus campylobacter CCFM1268 and Lactobacillus sake QJSNT1L10 respectively. Bacterial suspension CCFM1268, QJSNT1L10 intervention group. Among them, each mouse in the blank group and the model group was intragastrically administered with normal saline at a dose of 0.2 mL once a day, and each mouse in the other groups was intragastrically administered 1×10 at a dose of 0.2 mL 9 CFU / mL bacterial suspension. During the experiment, the blank group was given normal drinking water, and the other groups were given 2.5% DSS drinking water. After the experiment, each mouse was anesthetized with isoflurane and sacrificed. ...

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Abstract

The invention discloses lactobacillus curvatus capable of promoting generation of short-chain fatty acids in intestinal tracts and application of the lactobacillus curvatus, and belongs to the technical field of microorganisms and the technical field of medicines. The lactobacillus curvatus CCFM1268 provided by the invention can promote intestinal health, reduce the disease activity index of colitis mammals, relieve shortening of colon and reduce damage of colon tissue; the content of tight junction protein of colon tissue of a mammal with colitis is increased; (3) reducing the content of proinflammatory factors IL-6, IL-17, IL-1beta and TNF-alpha in the colon tissue of the mammal with colitis; (4) promoting the content of short-chain fatty acids in intestinal tracts of mammals with colitis; and (5) regulating intestinal flora structures of mammals with colitis. Therefore, the lactobacillus curvatus CCFM1268 has a huge application prospect in preparation of products (such as food or medicines) for protecting intestinal health.

Description

technical field [0001] The invention relates to a lactobacillus campylobacter capable of promoting the production of short-chain fatty acids in the intestinal tract and its application, belonging to the technical field of microorganisms and the technical field of medicine. Background technique [0002] Inflammatory bowel disease (IBD), mainly including Crohn's disease (CD) and ulcerative colitis (UC), is a gastrointestinal inflammation that threatens human health, and Its incidence is increasing year by year worldwide. CD primarily induces transmural inflammation of the gut and can affect any part of the gastrointestinal tract. It is clinically manifested as chronic recurrent onset and weight loss. In contrast, UC is mainly characterized by mucosal inflammation, limited to the colon, and clinically characterized by chronic or acute onset with weight loss and diarrhea. [0003] Studies have shown that the occurrence and development of inflammatory bowel disease may be rela...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P1/00A61P29/00A61P1/04A23L33/135A23L2/38A23C9/123A23L11/65A23L11/50A23C9/152A23C19/00A23G9/36C12R1/225
CPCC12N1/205C12N1/20A61K35/747A61P1/00A61P29/00A61P1/04A23L33/135A23L2/382A23C9/123A23C9/1238A23L11/65A23L11/50A23C9/152A23C19/00A23G9/363C12R2001/225A23V2002/00A23V2400/133A23V2400/123A23V2400/249A23V2200/3204Y02A50/30
Inventor 于雷雷翟齐啸田丰伟陈颖张程程陆文伟王刚崔树茂杨波毛丙永唐鑫张秋香赵建新张灏陈卫
Owner JIANGNAN UNIV
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