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Method for preparing optical pure phenylethanediol by utilizing microbial stereoselectivity transformation and its special-purpose microbe

A technology of pure phenylethylene glycol and biological method, applied in the field of biological separation of racemic compounds, can solve the problems of low optical purity of products

Inactive Publication Date: 2005-07-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0014] Kyoung et al. used naphthalene dioxygenase (NDO) to selectively oxidize styrene, but the optical purity of the obtained product was low, only 78.6% e.e.

Method used

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  • Method for preparing optical pure phenylethanediol by utilizing microbial stereoselectivity transformation and its special-purpose microbe
  • Method for preparing optical pure phenylethanediol by utilizing microbial stereoselectivity transformation and its special-purpose microbe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Whole-cell catalytic transformation of Candida parapsilosis C.parapsilosis CCTCC M203011:

[0087] In 2ml, 0.1mol / L potassium phosphate buffer solution (pH6.5), add 0.1g bacterial cells and 16mg racemic phenylethylene glycol, shake and react on a constant temperature shaker at 33°C for 48 hours, after the reaction , the mixture was centrifuged. The product (S)-PED had an optical purity of 98.80% e.e. and a yield of 92.86%.

Embodiment 2

[0089] Whole-cell catalytic transformation of Bacillus polymyxa CCTCC M203010:

[0090] In 2ml, 0.1mol / L potassium phosphate buffer solution (pH7.0), add 0.16g bacterial cells and 10mg racemic phenylethylene glycol, shake and react on a constant temperature shaker at 33°C for 60 hours, after the reaction , the mixture was centrifuged. The optical purity of the product (S)-PED increased to 95.61% e.e., and the yield increased to 47.98%.

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Abstract

The present invention relates to a method for preparing optical pure phenyl ethanediol by utilizing microbial stereoselective conversion and its special-purpose microbe. It utilizes the screening process to obtain two strains of Candiba parapsilosis CCTCC M203011 and Bacillus polymyxa CCTCC M203010. Two strains can be used to make asymmetric conversion of racemic phenyl ethanediol so as to obtain the product (S)-phenyl ethanediol, its optical purity is higher. Besides, it also provides optimized culture medium composition, culture condition, reaction system and reaction condition.

Description

technical field [0001] The invention discloses a method for preparing optically pure phenylethylene glycol by stereoselective conversion of microorganisms and special microorganisms thereof, belonging to the technical field of biological separation of racemic compounds. Background technique [0002] Optically pure phenylethylene glycol, that is, (S)-phenylethylene glycol, also known as (S)-PED, is not only an indispensable and important chiral additive in liquid crystal materials, but also has become a As an important intermediate of medicine, pesticide and functional materials, it is very meaningful to carry out research on the resolution method of phenylethylene glycol. [0003] Chiral compounds play an important role in people's lives. Since the two enantiomers are different in pharmacology, toxicology and functional effects, the preparation of optically pure chiral modular compounds is very important in medicine, agriculture, materials and Environmental protection and o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/18C12P41/00
Inventor 徐岩王栋穆晓清聂尧
Owner JIANGNAN UNIV
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