Synthesis and apparatus for high-flux polypeptide
A peptide synthesis, high-throughput technology, applied in peptide preparation methods, chemical instruments and methods, peptides, etc., can solve the problem of peptide quantity and quality decline
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Embodiment 1
[0058] Example 1 Using the first process condition to synthesize 17 polypeptides with the same length of peptide chain, the same first amino acid at the C-terminal and different sequences
[0059] General Fmoc solid-phase synthesis techniques and steps are applicable here. Although all amino acids (including the D-form) are suitable for use herein, only the L-form is used in this example. The degree of substitution of all amino acid carriers 7 was controlled at about 0.4mmol / g. The used solvents such as DMF and coupling agents such as HBTU are conventional.
[0060] The 17 polypeptides contain the same C-terminal amino acid and different peptide chain lengths, and their polypeptide sequences are as follows (single-letter amino acid abbreviation):
[0061] First peptide A-L-A-A-G
[0062] Second peptide A-R-A-G
[0063] Third Peptide L-A-L-A-G
[0064] Quaternary peptide R-L-A-G
[0065] Fifth peptide R-A-R-A-G
[0066] Sixth peptide L-A-R-G
[0067] Seventh peptide R-L...
Embodiment 2
[0086] Example 2 Using the second process condition to synthesize 17 polypeptides with the same length of peptide chain, the same first amino acid at the C-terminal and different sequences
[0087] The polypeptide to be synthesized in this example and the operation process are exactly the same as those in Example 1, but the process conditions used are different.
[0088] 1), 2) step operation is exactly the same as embodiment 1.
[0089] 3) Put all the polypeptide reactors 2 together, and then carry out the reaction of removing the Fmoc protecting group. After 30 minutes, filter off the deprotecting group reagent, and put all the polypeptide reactors 2 into the polypeptide eluting device 3 and shake off Dry. Take out all the peptide reactors 2, wash them with DMF, put them into the peptide elutor 3 and dry them, and repeat this 4 times. According to the instructions of the peptide chip 1, all the second amino acids containing the C-terminal are A(Ala) The polypeptide reactor...
Embodiment 3
[0095] Example 3 Using the third process condition to synthesize 17 polypeptides with the same length of peptide chain, the same first amino acid at the C-terminal and different sequences
[0096] The polypeptide to be synthesized in this example and the operation process are exactly the same as those in Example 1, but the process conditions used are different.
[0097] 1), 2) step operation is exactly the same as embodiment 1.
[0098] 3) Put all the polypeptide reactors 2 together, and then carry out the reaction of removing the Fmoc protecting group. After 50 minutes, filter off the deprotecting group reagent, and put all the polypeptide reactors 2 into the polypeptide eluting device 3 and shake off Dry. Take out all the peptide reactors 2, wash them with DMF, put them into the peptide elutor 3 and dry them, and repeat this 6 times. According to the instructions of the peptide chip 1, all the second amino acids containing the C-terminal are A(Ala) The polypeptide reactor ...
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