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Disposable immobilized enzyme column suitable for agricultural fast detection

An immobilized enzyme, one-time technology, applied in the direction of enzymes, biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of insufficient regeneration technology, complicated immobilization procedures, etc., and achieve the effect of simple manufacture and low cost

Inactive Publication Date: 2006-03-01
UNIV OF SHANGHAI FOR SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there are also enzymes that use various effects on the sensing electrodes, due to the lack of regeneration technology and the complicated immobilization procedures of this technology, it is only in an experimental state at present.

Method used

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  • Disposable immobilized enzyme column suitable for agricultural fast detection

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] 1. Extraction of enzyme solution

[0026] The pulverized coarse wheat flour of about 20 mesh was mixed with a buffer solution of pH 6.4 ionic strength 0.025M at a ratio of 5 L / 1 kg, and stirred at room temperature of 20° C. for 12 hours. The above extract was filtered with 100 mesh silk cloth. The supernatant obtained by centrifuging the above filtrate with a sedimentation centrifuge is the wheat crude enzyme liquid.

[0027] 2. Pretreatment of immobilized carrier

[0028] Carrier: Large pore size strongly basic anion exchange resin

[0029] (1) Resin is air-dried at room temperature;

[0030] (2) Sieve the dry resin with a standard sieve, and get the resin of the 40 mesh fraction for subsequent processing;

[0031] (3) Wet soak the dry resin with deionized water to make it fully swell;

[0032] (4) Carry out centrifugal dehydration to the fully swollen resin with centrifuge;

[0033] (5) Add 0.5M NaOH solution to the wet resin at a ratio of 1.5L / kg, stir at room ...

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Abstract

A disposable immobilized enzyme column for quickly detecting agricultural chemicals is prepared through extracting enzyme liquid from wheat through breaking wheat, immersion extracting, filtering and centrifugal separating, pretreating, carrier through pretreating ion exchange resin, stirring, adsorption and washing to obtain anionic exchange resin, immobilizing enzyme through mixing said enzyme liquid with said resin, stirring, washing, dewatering, and adding buffering liquid, and loading it in column shell.

Description

technical field [0001] The invention relates to a disposable immobilized enzyme column suitable for rapid detection of pesticides, which belongs to the field of rapid detection of pesticides. Background technique [0002] At present, there are many technologies for rapid determination of pesticide residues. Most of them are based on the inhibition of specific enzyme reactions by pesticides. In terms of reaction systems, some use liquid enzymes to react, and some use immobilized enzymes immobilized on paper carriers for reactions. But for the use on the instrument, the purpose is to measure the granular immobilized enzyme preparation or enzyme column of the reaction solution product signal and have not yet come out. Although there are also enzymes that use various effects on the sensing electrodes, due to the lack of regeneration technology and the complicated immobilization procedures of this technology, it is only in the experimental state at present. Contents of the in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/00C12Q1/00
Inventor 许学勤徐斐华泽钊
Owner UNIV OF SHANGHAI FOR SCI & TECH