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Method for tissue culturing gastodia tuber

A tissue culture and gastrodia elata technology, applied in the field of plant tissue culture, can solve the problems such as the limited effect of increasing the weight and volume of Gastrodia elata, the increase in the quantity of Gastrodia elata, etc., and achieve the effects of simple equipment and promoting growth and proliferation.

Inactive Publication Date: 2006-03-15
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many difficulties in the factory production of Gastrodia elata
[0004] Plant Physiology Communications, 1996, 32 (3), Liu Nengjun "In Vitro Culture of Gastrodia elata"; Chinese Herbal Medicine, 2001, 32 (5), Cai Yongping et al. Proliferation, but the Gastrodia elata cultivated by them only increases in quantity, the effect of increasing the weight and volume of Gastrodia elata is limited, and the individual weight generally does not exceed 10g, which cannot be regarded as a complete Gastrodia elata tissue culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Medium 1 is: 1 / 2MS+6-BA 2mg / L+NAA 0.05mg / L+Glu 10mg / L+Asp 5mg / L.

[0018] White hemp is cultivated for 28 days to form new rice hemp. Separating millet hemp is aseptically transferred to medium 1, millet hemp begins to grow after 27 days, can grow into small white hemp of 3-5cm after 55 days, can grow into big white hemp of 10-15g after 150 days.

Embodiment 2

[0019] Example 2 Medium 2 is: 1 / 2MS+6-BA 2mg / L+NAA 0.05mg / L+Glu 100mg / L+Asp 5mg / L.

[0020] White hemp is cultivated for 23 days to form new rice hemp. Separating millet hemp is aseptically transferred to medium 2, millet hemp begins to grow after 22 days, can grow into small white hemp of 3-5cm after 51 days, can grow into big white hemp of 10-20g after 150 days.

Embodiment 3

[0021] Example 3 Medium 3 is: 1 / 2MS+6-BA 2mg / L+NAA 0.05mg / L+Glu 1000mg / L+Asp 5mg / L.

[0022] White hemp is cultivated for 22 days to form new rice hemp. Separating millet hemp is aseptically transferred to medium 3, millet hemp begins to grow after 22 days, can grow into small white hemp of 3-5cm after 53 days, can grow into big white hemp of 10-18g after 150 days.

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PUM

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Abstract

An efficient tissue culture method of gastrodia includes taking the living gastrodia from field, washing, disinfecting with medical alcohol, bleach powder and HgCl2, flushing with aseptic water 4-6 times, cutting its lender stem into blocks by 0.5-1 cm, inoculating it in the culture meidum containing asparagic acid and glutamic acid or sodium glutamate, and culturing at 25+ / -2 deg.c.

Description

Technical field [0001] The invention relates to a plant tissue culture method, in particular to a Gastrodia elata tissue culture method. Background technique [0002] Gastrodia elata Bl. is a kind of Orchidaceae plant with degenerated roots and leaves, and is a well-known traditional Chinese medicine. Sweet in taste, flat in nature; calming the liver and relieving wind, dispelling wind and relieving convulsions, it is a good medicine for dizziness, numbness of limbs, convulsions in children, epilepsy, hypertension, and ear-induced vertigo. my country is the main producing area of ​​Gastrodia elata. Due to the continuous increase of demand and the decrease of wild drug sources, the supply is in short supply. In order to meet the market demand, it will be the key to solve the problem to be able to cultivate it in a factory. [0003] Gastrodia elata itself lacks the ability to assimilate external elements and obtain mineral nutrition. Most of the external mineral nutrition is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 吴沿友刘能俊李萍萍
Owner JIANGSU UNIV