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Efficient drought-resisting and high phoshpate-tolerant nutritious bush mycorrhizal fungus and its production process

A technology for arbuscular mycorrhizal fungi and phosphorus nutrition, which is applied in the field of arbuscular mycorrhizal fungus Glomus mossii and its potted plants in greenhouses, and can solve the problem of short preservation time of mycelium and infected root segments, and easy contamination by field culture methods. , high cost and technical requirements, to achieve reliable practical application value, improve plant nutrient absorption, and improve the effect of drought resistance

Inactive Publication Date: 2006-03-15
北京市农林科学院植物营养与资源研究所 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nutrient thin layer method and the static water culture method are not used because of the small amount of spores produced and the short storage time of mycelia and infected root segments; the double aseptic culture method and glass bead culture method can obtain pure fungal materials with a relatively small amount , the operation and technology are difficult, and it is more suitable for scientific research and special purpose needs; the field culture method is easy to be polluted, and can be self-produced and used in some places in the season, and is not suitable for commercial bacterial agents; currently used for commercial bacterial agent production. The main method is the pot culture method, and the aerosol culture method can also be used, but the cost and technical requirements are high, so it is rarely used

Method used

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  • Efficient drought-resisting and high phoshpate-tolerant nutritious bush mycorrhizal fungus and its production process
  • Efficient drought-resisting and high phoshpate-tolerant nutritious bush mycorrhizal fungus and its production process

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Experimental program
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Effect test

Embodiment 1

[0055] The cultivation of embodiment 1 Glomus mosseae (Glomus mosseae) 93-6

[0056] Sorghum (Sorghum vulgare Pers., Aoza No. 1) was used as the host for bacterial agent production. The bacterial species is the Glomus mosseae 93-6 bacterial strain screened by the present invention, and the soil-sand mixture containing the spores of the bacterial strain, the infected root segment and the hyphae outside the root obtained by using sorghum as the host for expansion and propagation is used as the inoculation. agent. The culture substrate is a mixture of zeolite sand (volume ratio 1:1), in which the river sand is collected from the Yongding Riverbed near the Lugou Bridge in Beijing and passed through a 2mm sieve. The zeolite was purchased from agricultural clinoptilolite at the Zeolite Factory in Dushikou Township, Hebei Province, with a particle size of 1.2 mm. Its basic physical and chemical properties: organic matter 0.366%, total phosphorus 0.024%, total potassium 1.88%, avail...

Embodiment 2

[0057] Embodiment 2 The application example of Glomus mosseae (Glomus mosseae) 93-6 in tomato cultivation

[0058] Peat and vermiculite were mixed at a volume ratio of 2:1 as the seedling culture substrate. Atmospheric pressure steam intermittently sterilized three times, 1 hour each time. The nutrient status of the substrate is shown in Table 1. The tomato variety used in the test was Jiafen 2. The test strain was Glomus mosseae 93-6 obtained from potted sorghum. Tomato seeds were sterilized by soaking in 40% formaldehyde 1:100 aqueous solution for 25 minutes, and then germinated at 25° C. for 48 hours for later use. The 72-hole seedling trays were washed with water, scrubbed with 75% alcohol, filled with sterilized seedling stage culture medium, and 1 g of inoculum was added to each hole. Finally, 3 tomato seeds were sown in each hole (2 plants were thinned out after emergence), covered with vermiculite and watered to saturation. After the seedlings are established, the...

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Abstract

The present invention relates to Glomus mosseae 93-6 as one kind of efficient drought-resisting and high phosphate-tolerant nutrients bush mycorrhizal fungus and its production process. The Glomus mosseae preparation is prepared with kaoliang as host plant, mixture of zeolite and sand as culture medium. The dried preparation contains 40-65 spores in each ml. Glomus mosseae may be grown in various kinds of plants to improve nutrients absorption, raise drought resistance and promote growth of plant. Test shows that the present invention has excellent seedling strengthening and yield increasing effect on straw berry, rice, water melon and other crops.

Description

technical field [0001] The invention relates to special microbial strain resources, and more specifically relates to an arbuscular mycorrhizal fungus Glomus mosseae with high efficiency of drought resistance and high phosphorus nutrition tolerance and a greenhouse potted production method thereof. Background technique [0002] Mycorrhizae, a symbiont formed by fungi and plant roots that are ubiquitous in nature, are not only of great significance in natural ecosystems, but also have a significant effect on promoting the growth and development of plants. Arbuscular mycorrhiza is the most common and most numerous type of mycorrhizal, and most crops form arbuscular mycorrhiza. A large number of scientific studies have shown that the existence of arbuscular mycorrhiza expands the absorption area of ​​plant roots, improves the absorption capacity of nutrients (especially phosphorus), enhances the stress resistance and disease resistance of plants, and promotes the formation of so...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01H17/00C12R1/645
Inventor 王幼珊陈宁张美庆李晓林邢礼军倪小会冯固张旭红
Owner 北京市农林科学院植物营养与资源研究所
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