Active peptide feed additive and its preparation method and use

A feed additive and active peptide technology, which is applied in the field of active peptide feed additive and its preparation, can solve the problems of allergic reaction to antigens, high preparation cost, difficult to popularize and use, and insignificant efficacy.

Active Publication Date: 2006-07-05
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the large amount of active peptide feed additive prepared in the prior art, the product function is not clear, the efficacy is not obvious, the peptide molecular weight is large and still has antigenicity, it is easy to cause antigen allergic reaction, and the preparation cost is high and it is not easy The disadvantages of popularizing and using in the animal breeding industry, so as to provide a feed with low addition amount and clear function, which can improve the absorption and utilization of nutrients (such as protein, minerals, vitamins, etc.) by animals, and increase the output of milk, meat and eggs. and production capacity, improve the quality and flavor of meat, eggs, and milk, stimulate the development of animal immune organs, improve disease resistance and reduce morbidity, and can significantly improve the water quality of aquatic animal breeding, increase the growth rate and survival rate of aquatic animals, and are widely used Active peptide feed additive for poultry, cattle, sheep, pigs, aquatic products and economic animals, which can be used as a substitute for antibiotics

Method used

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  • Active peptide feed additive and its preparation method and use
  • Active peptide feed additive and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1, prepare active peptide feed additive L-1 (liquid formulation)

[0074] The Bacillus subtilis was inserted into the sterilized liquid medium, and cultured at 25° C. for 48 hours to obtain the seed liquid.

[0075] The liquid culture medium is a mixed solution prepared in the following proportions: 1.3g of peptone, 3g of glucose, 3g of molasses, 50ml of potato infusion, 10g of soluble starch, 10g of sucrose, 2g of beef extract, 3.5g of yeast extract powder, soybean meal powder (60 mesh) 1.5g, growth factor 1ml, water 1000ml; the growth factor contains MgSO 4 .7H 2 O 20.00mg / ml, CaCl 2 .2H 2 O 2.20mg / ml, ZnSO 4 .7H 2 O 0.81mg / ml, FeSO 4 .7H 2 O 0.44mg / ml, MnSO 4 .H 2 O 0.13mg / ml, CuSO 4 .5H 2 O 0.02 mg / ml mixture.

[0076] The fermentation substrate - soybean protein powder is crushed to 60 meshes and then put into the fermenter, water and carbon source - cornmeal and molasses are added in turn (weight ratio is 1:1), the weight of the added water ...

Embodiment 2

[0091] Embodiment 2, prepare active peptide feed additive L-2 (liquid formulation)

[0092] Inoculate Aspergillus niger into the sterilized liquid medium, and culture at 35° C. for 24 hours to obtain seed liquid.

[0093] The liquid culture medium is a mixed solution prepared in the following proportions: 2.5g of peptone, 5g of glucose, 5g of molasses, 100ml of potato infusion, 20g of soluble starch, 30g of sucrose, 3g of beef extract, 5g of yeast extract powder, 2g of soybean meal, Growth factor 2ml, water 1000ml; the growth factor contains MgSO 4 .7H 2 O 30.00mg / ml, CaCl 2 .2H 2 O3.50mg / ml, ZnSO 4 .7H 2 O 1.10mg / ml, FeSO 4 .7H 2 O 0.60mg / ml, MnSO 4 .H 2 O 0.20mg / ml, CuSO 4 .5H 2 O 0.04 mg / ml mixture.

[0094] Fermentation substrate-soybean meal is crushed to 120 meshes and then put into the fermenter, water and carbon source-corn molasses and white sugar (weight ratio=3:2) are added successively, the weight of the added water is the same as that of the fermentati...

Embodiment 3

[0095] Embodiment 3, prepare active peptide feed additive L-3 (liquid formulation)

[0096] Inoculating Saccharomonas viridans into the sterilized liquid medium and culturing at 27° C. for 36 hours to obtain seed liquid.

[0097] The liquid culture medium is a mixed solution prepared in the following proportions: peptone 1.8g, glucose 4g, molasses 4g, potato extract 70ml, soluble starch 15g, sucrose 20g, beef extract 2.5g, yeast extract powder 4g, soybean meal powder (80 mesh) 1.8g, growth factor 1.5ml, water 1000ml; the growth factor contains MgSO 4 .7H 2 O 25.00mg / ml, CaCl 2 .2H 2 O 3.00mg / ml, ZnSO 4 .7H 2 O 1.00mg / ml, FeSO 4 .7H 2 O 0.50mg / ml, MnSO 4 .H 2 O0.17mg / ml, CuSO 4 .5H 2 O 0.03mg / ml mixed solution.

[0098] Put the fermentation substrate—bean cake into 80 meshes and put it into the fermenter, add water and carbon source—starch in sequence, the weight ratio of the weight of the water added to the protein contained in the fermentation substrate is 100:10,...

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Abstract

The present invention relates to an active peptide feed addictive, as a mixture of one or more plant protein and animal protein, which is the product of the following steps: fermenting and degrading the mixture through bacillus and the like, controlling the degree of hydrolysis at 20-35%, obtaining a fermentation supernatant after a separation post-processing process, concentrating drying the supernatant or directly adsorbing the supernatant onto the carrier, and then low temperature drying it. Said active peptide feed addictive is capable of obviously depressing development of bacillus coli and salmonella, and can prevention and cure diarrhea caused by bacilli or lienteric diarrhea; can exert its functions at a very low concentration, with a very intense effect; can regulate stomach and intestine bacterium group and promote animals digestion functions; can excite animal immune function, reinforce animal disease resistance against a plurality of epidemic diseases and greatly reduce the morbidity and death rate; can promote animal development and improve the growth velocity; can improve the feed use ratio and reduce production costs. Applications of the microorganism fermentation degradation method provided by the present invention have a low fabricating cost, and the obtained peptides have many kinds and high content, without environment pollutions.

Description

field of invention [0001] The invention relates to an active peptide feed additive, in particular to an active peptide (or oligopeptide) feed additive made from animal and vegetable proteins and a preparation method thereof. Background technique [0002] Since the 1950s, antibiotics, chemically synthesized drugs, hormones, β-stimulants, heavy metals, sedatives and other growth-promoting health care agents have been widely used in animal feeding and feed, leading to the following problems: drugs in livestock, poultry and aquatic products The residues are serious, directly endangering human health; the quality of livestock and poultry products has declined, lacking market competitiveness and trust; bacterial resistance has increased, and drug-resistant strains have increased, threatening human safety; destroying the ecological environment, etc. Due to the many side effects of antibiotics, scientists have been looking for alternatives to antibiotics for the past 20 to 30 years....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00A23J3/34A23J3/04A23J3/14C12N9/50A23K10/12A23K10/20A23K10/22A23K10/24A23K10/26A23K10/30A23K10/33A23K10/37A23K20/147A23K20/163A23K20/28
CPCY02P60/87
Inventor 张日俊于长青
Owner CHINA AGRI UNIV
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