Application of chitosan for preparing helicobacter pylorus vaccine adjuvant

Abstract

The invention discloses a new usage of chitose on pharmacy field that is application of chitose for preparing Helicobacter pylori vaccine adjuvant. The invention proves that chitose can be used as mucous membrane adjuvant for Helicobacter pylori vaccine by animal experiment. The action mechanism of chitose used as mucous membrane adjuvant is that chitose has good biological specificity and immunocompetence, can enhance immunogenicity of antigen, and promote absorption of stomach and intestine mucous membrane for antigen and immunoreaction of mucous membrane local, adjust immunoreaction balance of TH1 and TH2, then brings its adjuvant effect into play. The invention also proves immunoloregulation effect for that Helicobacter pylori infects local of stomach mucous membrane by animal experiment.

Description

technical field

[0001] The present invention relates to the field of pharmaceutical application invention, in particular to the application of chitosan in the preparation of Helicobacter pylori vaccine adjuvant Background technique

[0002] Chitosan (Chitosan), also known as chitosan, the chemical name is polymerized β-(1,4)-2-amino-D-glucose, that is, deacetylation at the 2nd position of chitin to expose -NH 2 The product formed by the gene is a high-molecular-weight straight-chain polysaccharide with the molecular formula (C 6 h 11 NO 4 )n, decomposes at about 185°C. Its average molecular mass is 1MDa, and its molecular chain length is about 5000 units, but in practical applications, its molecular weight, that is, the degree of polymerization, varies with different needs. Chitosan is white or light yellow powder, insoluble in neutral and alkaline aqueous solutions and most organic solvents, soluble in monovalent inorganic acids and organic acids. Its solubility depend...

Examples

Embodiment 1

[0231] With known methods in the technical field, with 88.5% deacetylated chitosan as raw material, 88.5% deacetylated chitosan is dissolved in 0.8% acetic acid brine in a ratio of 3% to form chitosan acid solution, and then Chitosan acid solution is used as mucosal adjuvant, and Helicobacter pylori antigen is used to prepare a vaccine for the prevention and treatment of Helicobacter pylori infection.

Embodiment 2

[0233] Using methods known in the art, using 88.5% deacetylated chitosan as raw material, 88.5% deacetylated chitosan and physiological saline are made into a 10 mg / ml solution, and then treated with ultrasound (output power 80HZ) for 2 Once, every 5 minutes, with an interval of 1 minute, get its supernatant after centrifuging at 50g for 10 minutes, and filter it with a 400 mesh screen, then collect its sediment after centrifuging at 1400g for 10 minutes, which is small chitosan particles. Then the small chitosan particles are used as mucosal adjuvant, and Helicobacter pylori antigen is used to prepare a vaccine for the prevention and treatment of Helicobacter pylori infection.

technical field

[0001] The present invention relates to the field of pharmaceutical application invention, in particular to the application of chitosan in the preparation of Helicobacter pylori vaccine adjuvant Background technique

[0002] Chitosan (Chitosan), also known as chitosan, the chemical name is polymerized β-(1,4)-2-amino-D-glucose, that is, deacetylation at the 2nd position of chitin to expose -NH 2 The product formed by the gene is a high-molecular-weight straight-chain polysaccharide with the molecular formula (C 6 h 11 NO 4 )n, decomposes at about 185°C. Its average molecular mass is 1MDa, and its molecular chain length is about 5000 units, but in practical applications, its molecular weight, that is, the degree of polymerization, varies with different needs. Chitosan is white or light yellow powder, insoluble in neutral and alkaline aqueous solutions and most organic solvents, soluble in monovalent inorganic acids and organic acids. Its solubility depend...

Examples

Embodiment 1

[0231] With known methods in the technical field, with 88.5% deacetylated chitosan as raw material, 88.5% deacetylated chitosan is dissolved in 0.8% acetic acid brine in a ratio of 3% to form chitosan acid solution, and then Chitosan acid solution is used as mucosal adjuvant, and Helicobacter pylori antigen is used to prepare a vaccine for the prevention and treatment of Helicobacter pylori infection.

Embodiment 2

[0233] Using methods known in the art, using 88.5% deacetylated chitosan as raw material, 88.5% deacetylated chitosan and physiological saline are made into a 10 mg / ml solution, and then treated with ultrasound (output power 80HZ) for 2 Once, every 5 minutes, with an interval of 1 minute, get its supernatant after centrifuging at 50g for 10 minutes, and filter it with a 400 mesh screen, then collect its sediment after centrifuging at 1400g for 10 minutes, which is small chitosan particles. Then the small chitosan particles are used as mucosal adjuvant, and Helicobacter pylori antigen is used to prepare a vaccine for the prevention and treatment of Helicobacter pylori infection.

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