Mucous membrane immunizing agent of polyglycol-inactivated vaccine
A technology of polyethylene glycol and mucosal immunity, which is applied in the direction of antiviral agents, viral antigen components, antibody medical components, etc., can solve problems such as adverse reactions, and achieve the effects of enhancing immunogenicity, improving absorption, and prolonging residence time
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0015] Example 1 Concentration of polyethylene glycol to inactivate SARS coronavirus
[0016] The pharmaceutical grade polyethylene glycol 6000 is formulated into 35% polyethylene glycol / 1.75M NaCl. Add the inactivated SARS coronavirus (CGMCC No.0977) venom in the amount of 1:5, and incubate at 4°C for 24 hours. Centrifuge at 13,000 rpm for 30 minutes. The supernatant was removed, and the precipitate was dissolved in phosphate buffer (0.01M PBS, PH7.2) to concentrate the virus to 100 times to obtain a crude inactivated SARS coronavirus mucosal immune preparation.
Example Embodiment
[0017] Example 2 Polyethylene glycol concentrates, inactivates and cracks influenza A virus
[0018] Pharmaceutical grade polyethylene glycol 8000 is formulated into 30% polyethylene glycol / 1.75M NaCl. Add the inactivated lysed influenza A virus solution in a ratio of 1:4, and incubate the H3N3 Panama strain (a domestically used production strain in 2003) at 4°C for 24 hours. Centrifuge at 13,000 rpm for 30 minutes. The supernatant is removed, and the precipitate is dissolved in phosphate buffer (0.01M PBS, PH7.2) to concentrate the virus to 50 times to obtain a crude inactivated avian influenza virus mucosal immune preparation.
Example Embodiment
[0019] Example 3 Polyethylene glycol enhances the immunogenicity of nasal drops SARS coronavirus vaccine
[0020] SARS coronavirus (G250) inactivated vaccine, immunization dose: 50μg per mouse; PEG-SARS coronavirus inactivated mucosal immune preparation, immunization dose: 20μg per mouse, immunized Balb / c mice through nasal drops, respectively. The immunization was performed every 2 weeks for a total of 4 immunizations. The mice were sacrificed 2 weeks after the fourth strong immunization, blood was taken to separate the serum, and the trachea and lungs of the mice were lavaged with phosphate buffer. Serum and tracheal-lung lavage fluid were stored at -70°C for testing. Serum is used to detect anti-SARS coronavirus neutralizing antibodies; tracheal-lung lavage fluid is used to detect anti-SARS coronavirus IgA (indirect immunofluorescence method). The results showed that the immunity of the PEG-inactivated vaccine nose drops was significantly higher than that of the inactivated vac...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap