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Mixed bed ion exchange resin method for purifying lecithin and cephalin

A technology of ion exchange resin and lecithin, which is applied in the field of phospholipids, can solve the problems that the application of natural products has not yet been launched, and achieve the effects of low cost, convenient operation and high purity

Inactive Publication Date: 2006-10-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although mixed bed ion exchange resins have been successfully used in the preparation of deionized water, their application in the separation and purification of natural products has not yet been developed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Fresh egg yolk is degreased three times with acetone, extracted twice with 95% ethanol, concentrated and dried in vacuum to obtain crude phospholipids. Through HPLC analysis, the crude phospholipid contains PC76.7% (wt%) and PE15.7% (wt%). Take 17.5ml of pretreated strong acidic resin and 52.5ml of pretreated strong basic resin and mix them evenly, then wet-install the Φ2.7ID×40cm column, first wash 500ml with deionized water, and then wash 300ml with methanol to replace the resin in the resin. water. Inject 100ml of crude phospholipid methanol solution with a concentration of 100mg / ml, rinse with 250ml of methanol, and control the flow rate at 1 to 1.5ml / min. After adding the sample, start to collect the effluent (collect about 25ml per part); (volume percentage) methanol solution of ammoniacal liquor (containing 25% by weight of ammonia) flushes 300ml, and the same flushing flow rate is controlled at 1~1.5ml / min, and is collected by about 25ml in every part. The eff...

Embodiment 2

[0026] The preparation of crude phospholipids is the same as in Example 1. Take 14ml of the pretreated strong acidic resin and 56ml of the pretreated strong basic resin and mix them evenly, then wet install the Φ2.7ID×40cm column, first wash 500ml with deionized water, and then wash 300ml with methanol to replace the water in the resin. Inject 100ml of crude phospholipid methanol solution with a concentration of 100mg / ml, rinse with 250ml of methanol, and control the flow rate at 1 to 1.5ml / min. After adding the sample, start to collect the effluent (collect about 25ml per part); (volume percentage) methanol solution of ammonia water (containing 25% ammonia by weight percentage) flushes 200ml, and the same flushing flow rate is controlled at 1~1.5ml / min, and is collected by about 25ml in every part. The effluents were combined after TLC analysis, concentrated in vacuo and freeze-dried to obtain 3.4 g of lecithin (purity 90%) and 1.5 g of cephalin (purity 55%). The column can ...

Embodiment 3

[0028] The preparation of crude phospholipids is the same as in Example 1. Take 23.3ml of the pretreated strong acidic resin and 46.7ml of the pretreated strong basic resin and mix them evenly, then wet-install the Φ2.7ID×40cm column, first wash 500ml with deionized water, and then wash 300ml with methanol to replace the resin in the resin water. Inject 100ml of crude phospholipid methanol solution with a concentration of 100mg / ml, rinse with 250ml of methanol, and control the flow rate at 1 to 1.5ml / min. After adding the sample, start to collect the effluent (collect about 25ml per part); then use 3.5% (volume percentage) methanol solution of ammonia water (containing 25% by weight of ammonia) flushes 250ml, and the same flushing flow rate is controlled at 1~1.5ml / min, and is collected by about 25ml in every part. The effluents were combined after TLC analysis, concentrated in vacuo and freeze-dried to obtain 4.0 g of lecithin (89% purity) and 1.6 g of cephalin (58% purity)....

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PUM

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Abstract

The invention discloses a method for refining lecithin and cephalin with mixed bed ion exchange resin. Its steps are as follows: 1) the egg yolk is degreased with acetone, extracted with 95% ethanol, then concentrated in vacuum and dried in vacuum to obtain crude phospholipid; 2) the pretreated strong acid resin and strong base resin are evenly mixed and then wet layered To analyze the column, first wash with deionized water, and then rinse with methanol to replace the water in the resin; 3) Add the crude phospholipid sample solution dissolved in methanol to the column, and start collecting the effluent; 4) Rinse the column with methanol, and collect the effluent 5) rinse the column with ammonia-containing methanol solution, and collect the effluent; 6) the effluent is combined after thin-layer chromatography TLC analysis, and after vacuum concentration and freeze-drying, lecithin with a purity greater than 92% and a purity greater than 80% can be obtained respectively. % cephalin; 7) the column is washed and then injected. The process of the invention is simple and easy to operate; the resin can be recycled and the cost is low; the resin column chromatography process can be carried out at room temperature; and the product has high purity.

Description

technical field [0001] The invention relates to phospholipids, in particular to a method for refining lecithin and cephalin with a mixed bed ion exchange resin. Background technique [0002] Lecithin is a phospholipid widely distributed in animals and plants. Its chemical name is Phosphatidyl Choline (PC for short). It is the main source of choline and essential fatty acids in the human body and plays an important role in maintaining human health. effect. At the same time, lecithin is a natural emulsifier with unique functions such as emulsification, penetration, moisturizing and anti-oxidation. It is widely used in food, health care products, daily chemicals, medicine, feed, leather and other fields. Cephalin is another important phospholipid, and its chemical name is Phosphatidylethanolamine (Phosphatidyl Ethanolamine, referred to as PE). Lecithin and cephalin have been widely used in food, medicine, daily necessities and other industries, especially high-purity phosphol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F9/10
Inventor 吕秀阳
Owner ZHEJIANG UNIV