Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing water-soluble free amine chitosan

A technology of chitosan and free amine, which is applied in the field of preparing high-purity water-soluble free amine chitosan, and can solve the problems of no description, no implied characteristics, etc.

Inactive Publication Date: 2006-12-13
郑特来
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above disclosure neither describes nor suggests the properties of chitosan having high purity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing water-soluble free amine chitosan
  • Method for preparing water-soluble free amine chitosan
  • Method for preparing water-soluble free amine chitosan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] refer to figure 1 , the water-soluble free amine chitosan (500) was prepared by reacting chitosan oligosaccharate (100) as an initial raw material with a trialkylamine, followed by adding an organic solvent to remove the acid salt (200); addition of mineral acid to remove trialkylamine at C-6 position (300); and purification (400).

[0023] In step (a), the reaction is carried out by reacting chitosan oligosaccharate with a trialkylamine in phosphate buffered saline. The resulting reaction mixture is then dissolved in a suitable organic solvent, followed by work-up to remove the acid salt.

[0024] In a preferred embodiment, chitosan oligosaccharates are prepared as starting materials by dissolving chitosan in a suitable acid. This is followed by known enzymatic methods, whereby the resulting chitosan solution is treated by known enzymes. Enzymes derived from microorganisms to convert chitosan oligosaccharides may include chitosanase from Bacillus pumilus, Bacillus s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A method for the preparation of water-soluble chitosan with high purity and biological activity includes steps of: reacting chitosan oligo sugar acid salt, covalently bonded to an organic or inorganic acid, with trialkylamine in phosphate buffered saline followed by addition of an organic solvent to remove acid salt at C-2 position; adding the thus obtained reaction mixture to an inorganic acid to remove trialkylamine salt at C-6 position; and passing the thus obtained free amine chitosan through an activated carbon / ion exchange resin. The free amine chitosan is water-soluble and has a high bioavailability for application to the medicine and food industries.

Description

field of invention [0001] The invention relates to a method for preparing high-purity water-soluble free amine chitosan. Background of the invention [0002] Chitosan is a highly insoluble N-acetylated polymer of β-(1,4)-D-glucosamine with pyranose units and a high molecular weight biopolymer with over 1 million Daltons, the biological The polymer is bonded to functional groups of over 5,000 glucosamine units. Chitosan is a cellulosic polymer found in the walls of fungi and the exoskeletons of arthropods including insects, crabs, shrimps and lobsters. Chitosan, which has structural units similar to cellulose, can be applied in many fields of pharmaceutical industry due to its high bioavailability and negative effect on immune response. Since the FDA (USA) recognized chitosan as a food additive, chitosan has been used in bioengineering as an important biomedical material. [0003] For example, chitosan can be used in a variety of industrial applications, including wastewat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
CPCC08B37/003
Inventor 罗在云郑特来张美卿崔昌镛金元锡孔炳起丁映日梁铉弼张智泰
Owner 郑特来