Breeding process of transgenic bollworm resisting cotton hybrid

A technology for hybrids and cotton bollworms is applied in the directions of plant genetic improvement, botanical equipment and methods, applications, etc., which can solve the problems of increasing cotton planting costs, poor chemical control effect, and farmland ecological environment pollution, etc., to improve breeding efficiency, The effect of improved agronomic traits and shortened life span

Inactive Publication Date: 2002-10-16
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

Chemical control less effective as bollworm resistance grows
Extensive use of chemical pesticides to prevent and control cotton bollworm not only inc

Method used

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  • Breeding process of transgenic bollworm resisting cotton hybrid
  • Breeding process of transgenic bollworm resisting cotton hybrid
  • Breeding process of transgenic bollworm resisting cotton hybrid

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Experimental program
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Embodiment

[0027] The original basic material "SGK321" of the Bt+CpTI gene insect-resistant cotton issued by the Biotechnology Center of the Chinese Academy of Agricultural Sciences utilized by the present invention has general economic properties (only about 70 kilograms per mu), and insect resistance exists between strains (purity is about 95%). For this purpose, individual plant selection and progeny identification were carried out on this material. Individual plants and strains homozygous for resistance to cotton bollworm were selected, and various methods were adopted for resistance identification.

[0028] Kanamycin marker identification: Tear the absorbent cotton into small strips, dip it in 500ppm kanamycin solution, and stick it on the new leaves of the cotton plant. After 5 days, the leaves of all susceptible cotton plants stained with kanamycin solution showed obvious yellow spots, while the leaves of Bt+CpTI gene-transferred insect-resistant cotton had no symptoms.

[0029]...

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Abstract

The present invention is the breeding process of transgenic bollworm resisting cotton hybrid, Bt+CpTI. To transgenic bollworm resisting cotton SGK321, bollworm resistance identification and breeding is performed to obtain transgenic bollworm resisting cotton SGK321' with bollworm resistance isozygoty of 100 % and yield 9.5% higher than the original variety. Bollworm resisting cotton hybrid Nankang-7 is further obtained by using as SGK321'as male parent and Sumian-12 as female parent and it has high yield and high bollworm resistance and can inhibit the resistance of bollworm to the cotton.

Description

Field of invention: [0001] The invention discloses a method for breeding transgenic cotton bollworm-resistant cotton hybrids, which belongs to cotton transgenic breeding methods and is specially used for the breeding of cotton bollworm-resistant cotton hybrids transgenic with Bt+CpTI bivalent genes. technical background: [0002] Cotton bollworm is the main pest that harms cotton, and it has caused great losses to cotton production in my country. With the enhancement of the resistance of cotton bollworm, the effect of chemical control is poor. Extensive use of chemical pesticides to control cotton bollworm not only increases the cost of cotton planting, increases the labor intensity of cotton farmers, but also causes pollution to the farmland ecological environment. Breeding insect-resistant cotton varieties is an important measure for the integrated control of cotton bollworm. The Bt+CpTI bivalent gene insect-resistant hybrid cotton has significant resistance to cotton bo...

Claims

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Application Information

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IPC IPC(8): A01H1/00A01H1/02
Inventor 张天真朱协飞唐灿明郭旺珍
Owner NANJING AGRICULTURAL UNIVERSITY
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