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Application of osteogenic growth peptide in promoting bematogenesis

A technology of osteogenic growth peptide and hematopoietic function, which is applied in the application field of osteogenic growth peptide in promoting hematopoiesis, and can solve the problems of no promotion, shortening the recovery time of leukocytes and neutrophils, and depletion of hematopoietic progenitor cells.

Inactive Publication Date: 2003-06-04
SHANGHAI YIZHONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

They directly stimulate the proliferation of myeloid hematopoietic progenitor cells, shorten the recovery time of white blood cells and neutrophils, and have high curative effect, but they are expensive and cannot be afforded by the working class
[0007] In addition, there are still some problems in the clinical use of rhG-CSF and rhGM-CSF: (1) Tumor and leukemia cells have normal receptors for CSF, which may increase the recurrence rate of tumors by increasing the proliferation of host residual tumor cells after administration; (2) ) CSF directly stimulates the proliferation of hematopoietic progenitor cells without self-renewal ability, leading to the exhaustion of hematopoietic progenitor cells; (3) selectively promotes the proliferation of granulocyte progenitor cells, but has no effect on erythroid and megakaryocyte lineage; (4) clinical It is necessary to closely monitor the changes of white blood cells to prevent the proliferation of immature cells

Method used

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  • Application of osteogenic growth peptide in promoting bematogenesis
  • Application of osteogenic growth peptide in promoting bematogenesis
  • Application of osteogenic growth peptide in promoting bematogenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Hematopoietic effect of osteogenic growth peptide in animal model of radiation injury

[0060] The purpose of this example is to observe the therapeutic effect of sOGP on leukopenia, and to find safe and effective drugs for the treatment of tumor patients with bone marrow damage caused by radiotherapy and chemotherapy.

[0061] method:

[0062] Clean grade ICR mice, ♂, weighing 18-20 g, were randomly divided into groups, 10 in each group. Two kinds of irradiation doses were used, and the experiment was carried out in two batches. The experimental groups are as follows:

[0063] Group A: normal control group, without irradiation;

[0064] Group B: irradiation control group;

[0065] Group C: Huier blood positive drug control group, 100 μg / kg / day;

[0066] Group D: OGP medication group, divided into 9 dose points,

[0067] (1) 0.00078nmol / mouse / day; (2) 0.00156nmol / mouse / day; (3) 0.00625nmol / mouse / day; (4) 0.025nmol / mouse / day; (5) 0.10nmol / mouse / day; (6) 0.40 nmol / ...

Embodiment 2

[0090] The effect of sOGP on the hematopoietic system of normal mice

[0091] The purpose of this example is to observe whether sOGP can promote the hematopoietic function of normal mice, and provide a basis for improving hematopoietic function while treating osteoporosis and fractures clinically in the future, so as to enhance immunity and improve the therapeutic effect.

[0092] method:

[0093] Clean grade ICR mice, ♂, weighing 18-20 g, were randomly divided into groups, 10 in each group. The experimental groups are as follows:

[0094] Group A: blank control group;

[0095] Group B: sOGP medication group, divided into 3 dose points,

[0096] (1) 0.02nmol / mouse / day;

[0097] (2) 0.10nmol / mouse / day;

[0098] (3) 0.50 nmol / mouse / day.

[0099] Normal mice underwent sc sOGP for 14 consecutive days, and the following indicators were detected: ①Peripheral blood: WBC, Plt, RBC ②BMNC count, ③Bone marrow cell differential count.

[0100] result:

[0101] 1. Ef...

Embodiment 3

[0106] Hematopoietic activity of osteogenic growth peptide in vitro

[0107] The purpose of this example is to observe whether sOGP can promote the colony formation of human bone marrow myeloid progenitor cells in vitro, so as to provide evidence for sOGP to promote the proliferation of hematopoietic cells.

[0108] method:

[0109] Bone marrow cell semi-solid colony culture method was used. Prepare the bone marrow cell suspension first, adjust the cell concentration to 1×10 with RPMI-1640 6 cells / ml, add different concentrations of sOGP and Wheeler’s blood to the culture system, at 37°C, 5% CO 2 After culturing under the condition for 11 days, count the number of CFU-G colonies under a microscope (more than 50 cells are 1 colony). The experiment was divided into three groups, negative control group (without sOGP and G-CSF), wheel blood (G-CSF) positive control group, and sOGP experimental group with different concentrations. Each experimental point was repeated 3-4 dishes....

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PUM

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Abstract

Osteogenic growth peptide (OGP) is one kind of polypeptide found in human and animal body, containing 14 amino acids and exhibiting two-way osteoplast regulating effect. Research of the present invention shows that OGP, except bone formation strengthening effect, is also one hematogenesis promoting factor capable of promoting the proliferation of hematopoietic cell with granulocyte as main component. The present invention provides the application of OGP in preparing medicine composition for promoting the proliferation of hematopoietic cell with granulocyte as main component and the method of in vitro promoting granular archeocyte growth. Unlike other stimulation factors, OGP has no proliferation promoting effect on human TF-1 and other leucosis cells.

Description

technical field [0001] The invention relates to the application of osteogenic growth peptide (Osteogenic Growth Peptide, OPG) in promoting hematopoiesis. Background technique [0002] Osteogenic Growth Peptide (Osteogenic Growth Peptide) is a 14-amino acid polypeptide that can promote the growth of bone cells discovered in humans and animals by Bab et al. in 1988. When the body is fractured or the bone marrow is injured, in addition to the local osteogenic reaction in the fracture or damaged bone marrow, there is also a systemic osteogenic reaction [Bab I, et al. (1985) Calcif Tissue Int.37: 551-555 ; Foldes J, et al. (1989) J Bone Miner Res. 4:643-646]. In-depth experimental studies have shown that the healed bone marrow tissue can release several factors that promote osteogenesis into the blood circulation, resulting in an enhanced osteogenic response throughout the body. It has been confirmed, separated and purified by research. One factor is called Osteogenic Growth Pep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/00A61P7/00C07K7/08C12N5/08
CPCC07K7/08A61K38/00A61P7/00
Inventor 程文英陈统一崔大敷陈红红施德源刘智慧邵云潮李默漪罗伟华徐萍岳麓
Owner SHANGHAI YIZHONG BIOTECHNOLOGY CO LTD
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