Tissue engineering corium and its preparation method

A tissue engineering and dermis technology, applied in skin transplantation, medical science, prosthesis, etc., can solve the problems of not achieving epidermal reconstruction, high price, slow vascularization, etc.

Inactive Publication Date: 2004-05-05
陕西艾尔肤组织工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main disadvantages are: lack of viable fibroblasts, which may delay dermal reconstruction; collagen in acellular xenogenic dermal matrices may cause immune reactions; commercially available products only achieve dermal replacement, not epidermal reconstruction, and must be covered with autologous or cultured epidermis Cell sheets; Alloderm is derived from cadaver skin, limited source and risk of spre...

Method used

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Embodiment Construction

[0012] Now in conjunction with experiment the present invention will be described in further detail:

[0013] 1. Acquisition and expanded culture of fibroblasts: Take the skin tissue after circumcision of newborns. It has been confirmed that the fibroblasts from this source have a low immune response and will not cause obvious rejection. After the obtained foreskins were disinfected with promethamine or alcohol, fibroblasts were cultured by enzyme separation method or tissue block method. After the cells were confluent, they were digested and passaged with enzymes, and expanded for culture. The specific process is:

[0014] Wash the sterilized material with 0.1M phosphate buffered saline (PBS) containing 1% penicillin / streptomycin, remove subcutaneous fat and muscle layer, cut the skin into strips, add protease (Dispase) 4U / ml, Digest at 37 °C for 60-120 min. Remove the epidermis, cut the dermal tissue into small pieces, add 625U / ml collagenase to digest at 37°C for 90-160 m...

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Abstract

A tissue-engineered epidermis is prepared from skin fibroblasts through obtaining the skin fibroblasts, preparing culture medium, amplifying culture, preparing extocytic matrix compound, preparing biologic scaffold, compounding the skin fibroblasts on the surface of biologic scaffold, and 3D culture. Its advantages are a certain elasticity and toughness, short culture time, and no obvious immunorejection reaction.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering of biological materials, and relates to a tissue engineering dermis and a preparation method thereof. Background technique [0002] In recent years, in the fields of medical cosmetology, trauma, burn surgery, etc., with the requirements for functional recovery and aesthetic considerations, it is far from enough to achieve epithelialization of some deep skin defects, and efforts must be made to explore and apply more Reasonable method, so that the skin graft area can finally achieve the same or similar results as full-thickness skin grafting. Dermal substitutes play an important role in the skin reconstruction process. They can enhance skin elasticity, flexibility and mechanical wear resistance after wound healing, reduce scar hyperplasia, control contractures, improve the success rate of epidermal grafting and improve the quality of healing. Live dermal substitutes containing fibroblas...

Claims

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Application Information

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IPC IPC(8): A61F2/10A61L27/00A61L27/60
Inventor 金岩张勇杰聂鑫王新文董蕊
Owner 陕西艾尔肤组织工程有限公司
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