Tissue engineering corium and its preparation method
A tissue engineering and dermis technology, applied in skin transplantation, medical science, prosthesis, etc., can solve the problems of not achieving epidermal reconstruction, high price, slow vascularization, etc.
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[0012] Now in conjunction with experiment the present invention will be described in further detail:
[0013] 1. Acquisition and expanded culture of fibroblasts: Take the skin tissue after circumcision of newborns. It has been confirmed that the fibroblasts from this source have a low immune response and will not cause obvious rejection. After the obtained foreskins were disinfected with promethamine or alcohol, fibroblasts were cultured by enzyme separation method or tissue block method. After the cells were confluent, they were digested and passaged with enzymes, and expanded for culture. The specific process is:
[0014] Wash the sterilized material with 0.1M phosphate buffered saline (PBS) containing 1% penicillin / streptomycin, remove subcutaneous fat and muscle layer, cut the skin into strips, add protease (Dispase) 4U / ml, Digest at 37 °C for 60-120 min. Remove the epidermis, cut the dermal tissue into small pieces, add 625U / ml collagenase to digest at 37°C for 90-160 m...
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