Method for separating flavone glycoside from boerhavia diffusa

A careful and separated technology, applied in the field of separating bioactive flavone glycosides with anti-osteoporosis activity as shown in structural formula 1, can solve the problem that the flavone glycoside galactopyranoside is not fully extracted, and the separation method is heavy and uneconomical And other issues

Inactive Publication Date: 2004-07-28
COUNCIL OF SCI & IND RES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] 2. Using methanol to reflux for 6 hours is not enough to completely extract the flavone glycoside 3-O-β-D-galactopyranoside
This separation method is tedious and uneconomical, and unacceptable for the industrial production of the flavone glycoside 3-O-β-D-galactopyranoside

Method used

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  • Method for separating flavone glycoside from boerhavia diffusa
  • Method for separating flavone glycoside from boerhavia diffusa
  • Method for separating flavone glycoside from boerhavia diffusa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The shade-dried powdery leaves (1 kg) were extracted in a Soxhlet extractor using a hot continuous extraction method with rectified alcohol for 32 hours. The rectified alcohol was evaporated under reduced pressure to obtain a black-green substance, and the extract was ground successively with hexane, chloroform and ethyl acetate. The remaining residue was dissolved in methanol and placed in the refrigerator overnight, and the solid was separated by filtration and crystallized with methanol to obtain the flavone glycoside 3-O-β-D-galactopyranoside (4.0 g).

Embodiment 2

[0068] Shade-dried powdery leaves (1 kg) were extracted using methanol in a Soxhlet extractor using a hot continuous extraction method for 32 hours. The methanol was evaporated under reduced pressure to give a black-green material, and the extract was triturated successively with hexane, chloroform and ethyl acetate. The remaining residue was dissolved in methanol and placed in the refrigerator overnight, and the solid was separated by filtration and crystallized with methanol to obtain the flavone glycoside 3-O-β-D-galactopyranoside (3.5 g).

Embodiment 3

[0070] Shade-dried powdery leaves (1 kg) were percolated with rectified alcohol (4 x 41) for 64 hours. The rectified alcohol was evaporated under reduced pressure to give a black-green substance, and the residue was triturated successively with hexane, chloroform and ethyl acetate. The remaining residue was dissolved in methanol and placed in the refrigerator overnight, filtered, and the solid was isolated and crystallized from methanol to give the flavone glycoside 3-O-β-D-galactopyranoside (2.8 g).

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Abstract

The present invention relates to a process of isolation of bioactive eupalitin (3- omicron - beta -D-galactopyranoside) of formula 1 possessing anti-osteoporosis activity by extraction of powdered leaves of Boerhavia diffusa, with better yield.

Description

technical field [0001] The invention relates to a method for isolating biologically active flavone glycoside (eupalitin) from yellow rhizome. More specifically, the present invention relates to a method capable of isolating a bioactive flavone glycoside (3-O-β-D-galactopyranoside) with anti-osteoporosis activity as shown in structural formula 1 with high yield, which is Achieved by extracting the powdery leaves of the yellow slender leaves. [0002] Background of the invention [0003] Huang Xixin Linn. (Syn. Boerhavia repens Linn., Boerhavia procumbens Roxb.; Family Nyctaginaceae), popularly known as "Punarnava", is an important rejuvenating drug in Ayurveda. It is widely distributed throughout India and is very lush during the rainy season. The aerial part then disappeared, but revived or germinated in the following year [Sivarajan, V.V. and Balachandran, I. Ayurvedic drugs and their plant sources; Oxford & IBH publishing Co. Ltd., New Delhi, 1994]. The dried and mat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H17/07
Inventor 迪帕克·蒙德基纳捷德利拉·拉姆·马纳汉斯拉凯什·莫里苏克德夫·斯瓦米·汉达
Owner COUNCIL OF SCI & IND RES
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