2,3,5,4'-tetrahydroxy diphenyl ethylene-2-1-beta-d-glucoside
A technology of tetrahydroxystilbene and glucoside, which is applied in the field of natural compounds and can solve problems such as expensive processes and unfavorable mass production
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Embodiment 1
[0013] Example 1: Preparation of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (method 1)
[0014] Take slices of medicinal material, add water to decoct 3 times (each 2h, 1h, 1h), filter, combine the filtrates, concentrate, add 95% ethanol to make the alcohol content 60%, let stand overnight, and filter. Add 70% ethanol to the precipitate, heat and wash in a 70°C water bath, filter, combine the filtrate and the previous filtrate, recover ethanol under reduced pressure until there is no ethanol smell, add chloroform to extract twice to remove fat-soluble impurities, take the water layer, and extract completely with n-butanol ( Detected by TLC, the developer is chloroform-methanol (80:20), 10% ethanol sulfate for color development, or 254nm ultraviolet light for inspection), the n-butanol layers were combined, and evaporated to dryness under reduced pressure. Obtain the extract containing stilbene glycoside as the main component. The yield is 3%-7%.
[0015] Dissolve the ex...
Embodiment 2
[0018] Example 2: Preparation of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (method 2)
[0019] Take slices of medicinal materials, add water to decoct 3 times (each 2h, 1h, 1h), filter, combine the filtrates, concentrate, add 95% ethanol to make the alcohol content 60%, let stand overnight, and filter. Add 70% ethanol to the precipitate, heat and wash in a 70°C water bath, filter, combine the filtrate and the previous filtrate, recover the ethanol under reduced pressure until there is no ethanol smell, add chloroform to extract twice to remove fat-soluble impurities, take the water layer, evaporate the chloroform, add water to dilute, Filter, and the filtrate is passed through the D101 macroporous adsorption resin that has been processed, successively rinsed with water and an aqueous solution containing 30% ethanol until the target component is completely eluted (detected by TLC), and the eluent containing the component is combined (by 30% ethanol). -50% eluent), evapora...
Embodiment 3
[0021] Example 3: Effects on serum lipids of experimental animals with hypercholesterolemia [1]
[0022] Male SD rats were randomly divided into normal group, model group, positive drug (fenofibrate) control group, test drug (2,3,5,4′-tetrahydroxystilbene-2-O-β- D-glucoside) low-dose group, test drug (2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside) high-dose group (the same below). Except the normal group, the other 4 groups were administered with fat emulsion for 3 consecutive weeks. From the second week onwards, different treatments were given to each group: the normal group and the model group were given intragastric administration of normal saline (2ml / day), the control group were given intragastric administration of fenofibrate (100mg / kg·day), and the low-dose drug group was given intragastric administration. Oral administration of 80 mg / kg·day, and 160 mg / kg·day of the test drug group were administered by intragastric administration for 2 consecutive weeks.
[0023] S...
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