Scale production process of nitrobacteria in liquid of high activity and high stability

A technology of nitrifying bacteria and high stability, applied in the field of technology, can solve the problems of commercialization, slow commercialization process, large-scale cultivation and storage technology of nitrifying bacteria, etc., to achieve the promotion of attachment growth, long storage time, continuous collection A large amount of effect

Inactive Publication Date: 2004-11-17
韩舞鹰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the large-scale cultivation and preservation technology of nitrifying bacteria has not been overcome, and its productization and commercialization have been progressing very slowly.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] The present invention will be described in further detail below in conjunction with embodiment:

[0014] The specific operation process is as follows:

[0015] (1) Prepare the aerobic fermenter. A large-scale plastic pool with a depth of about 1 meter is selected, and air pipes are evenly arranged at the bottom, and the air is supplied by an air pump. The gas passes through an air filtration system to minimize bacteria and impurities in the air. Ensure that the dissolved oxygen in the culture medium is not less than 6mg / l. Fill with 4 / 5 volume of distilled water.

[0016] (2) Prepare a soft fiber carrier. The material is a soft, porous and dense thin-layer chemical fiber (PVC fiber cotton) with a specification of 30×10×3cm (length×width×thickness), which is evenly arranged in the water body of the fermenter. The basic parameters of each carrier are measured as: Geometric surface area 840cm 2 , specific gravity 0.8±0.1kgm -3 , specific surface area ≥ 93.3m2 m -3 ...

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Abstract

The invention discloses a scale production process of nitrobacteria in liquid of high activity and high stability wherein the process comprises, arranging flexible fiber in aerobiosis fermentation apparatus, employing culture medium containing low molecular organic carbon to accelerate the attached growth of the nitrifying bacteria, supplementing low molecular organic carbon source and nitrogen source in time, carrying out timely elution to the nitrifying bacteria on the soft fiber, conserving the collection liquid with the condition of low resolving oxygen, low pH and sealing strip, detecting and loading into packaging appliance.

Description

Technical field [0001] The invention relates to a process, in particular to a large-scale production process of liquid nitrifying bacteria with high activity and high stability. The product is a microbial preparation (liquid) mainly used to improve the aquaculture water environment and landscape water environment. Background technique [0002] There are mainly two types of nitrifying bacteria in the water environment: Nitrosomonas and Nitrobacter, which can efficiently convert ammonia nitrogen and nitrite to nitrate, so they have been widely valued. Nitrifying bacteria are mainly used in two aspects, one is to relieve the toxic effects of ammonia nitrogen and nitrite on farmed animals and ornamental animals; the other is to perform nitrification (or nitrification process), which is conducive to the next step of denitrification process and effectively reduces the water body. Nitrogen load. Nitrifying bacteria are typical chemoautotrophic bacteria, which obtain energy from t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/08C12N1/20
Inventor 韩舞鹰刘飞
Owner 韩舞鹰
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