The invention relates to an active biological recombiner and a preparation method thereof. Raw materials of the recombiner comprise the following components, by weight: 0.3-08% of sake yeast liquid, 0.3-08% of streptomyces griseus liquid, 70-90% of rice bran and the balance of water. A maltose medium is utilized to culture the sake yeast liquid and the streptomyces griseus liquid; then the raw materials are mixed and fermented to obtain the active biological recombiner. Compared with a prior art, the active biological recombiner of the invention is cultured by using rice bran as a main inocula carrier and sake yeast and streptomyces griseus as main starters, can degrade natural organic pollutants in the environment, has advantages of low manufacture cost, high production power, long period of validity, strong stability, simple storage conditions, convenience for transportation, composite compatibility of different bacteria, large pollution resistance span and large environment tolerance elasticity.