Technology of producing morin aqueous extract and polysaccharide using large scale liquid submerged fermentation process
A technology of liquid submerged fermentation and water extract, which is applied in the direction of fermentation and bacteria, and can solve problems such as difficulty in becoming a stable source of industrial products and the scarcity of Phellinus
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[0023] Phellinus mycelium purchased from China Microorganism Culture Collection Center (strain number: 5.95) was inoculated in the slant culture medium, the culture temperature was 28° C., and the culture time was 180 hours. The fresh slant strains were inserted into a 500ml Erlenmeyer flask containing 100ml of culture medium, cultured on a shaker at 28°C for 100 hours, and the shaker speed was 150rpm / min. Then the obtained shake flask bacterial strain was transferred into a 500ml Erlenmeyer shake flask equipped with 100ml medium for expansion culture, and cultured on a shaker at 28° C. for 45 hours at a shaker speed of 150 rpm / min. Then transfer 2200ml of the strains enlarged in the triangular flask into a 100L primary seed tank with 50L seed medium, keep the tank temperature at 28°C, the tank pressure at 0.7kPa, the stirring rate at 100rpm / min, and the ventilation rate at 1:0.5v / v.m, fermentation time 45 hours. Transfer the primary seed tank strains into a 1500L fermenter ...
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