Technology of producing morin aqueous extract and polysaccharide using large scale liquid submerged fermentation process

A technology of liquid submerged fermentation and water extract, which is applied in the direction of fermentation and bacteria, and can solve problems such as difficulty in becoming a stable source of industrial products and the scarcity of Phellinus

Inactive Publication Date: 2004-12-22
敖宗华 +2
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] Due to the very scarce amount of Phellinus from natural sources, it is difficult to become a stable source of industrial products
There is no report on large-scale liquid submerged fermentation to produce Phellinus Phellinus water extract and polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Phellinus mycelium purchased from China Microorganism Culture Collection Center (strain number: 5.95) was inoculated in the slant culture medium, the culture temperature was 28° C., and the culture time was 180 hours. The fresh slant strains were inserted into a 500ml Erlenmeyer flask containing 100ml of culture medium, cultured on a shaker at 28°C for 100 hours, and the shaker speed was 150rpm / min. Then the obtained shake flask bacterial strain was transferred into a 500ml Erlenmeyer shake flask equipped with 100ml medium for expansion culture, and cultured on a shaker at 28° C. for 45 hours at a shaker speed of 150 rpm / min. Then transfer 2200ml of the strains enlarged in the triangular flask into a 100L primary seed tank with 50L seed medium, keep the tank temperature at 28°C, the tank pressure at 0.7kPa, the stirring rate at 100rpm / min, and the ventilation rate at 1:0.5v / v.m, fermentation time 45 hours. Transfer the primary seed tank strains into a 1500L fermenter ...

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PUM

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Abstract

A process for preparing the aqueous extract and polyose from the Sanghuang fungus in large scale by deep liquid fermenting includes slo slant shake-flask culture, enlarge culture, class-one seeding culture, fermenting culture, heating while stirring for 2-4 hr, press fitlering, concentrating, spray drying to obtain its aqueous extract or depositing in alcohol, dissolving deposit in water, and spray drying to obtain its polyose.

Description

technical field [0001] The invention relates to a large-scale liquid submerged fermentation process for producing phellinus water extract and polysaccharides. technical background [0002] The modern scientific name of Phellinus igniarius is Phellinus igniarius, also known as mulberry ear, needle cleft hoof, cleft hoof pintle hole fungus, etc. Phellinus has been recorded as a blind medicinal material in Chinese medicine. After the mid-1990s, researchers in South Korea and Japan found that the extract of Phellinus Phellinus had an excellent inhibitory effect on tumors and other neoplasms. In vitro experiments confirmed that Phellinus water extract can induce cancer cells to enter programmed death. In addition, Phellinus also has a strong inhibitory effect on cancer cell metastasis. What's more exciting is that the Phellinus extract is harmless to the human body, and even if it is given in large doses for a long time, it has no toxic or side effects on humans or experimenta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C12P19/04
Inventor 敖宗华符慧子邹锡良
Owner 敖宗华
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