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Zymogen agent for treating agricultural wastes and preparation method

A technology for agricultural waste and fermentation inoculum, applied in the field of fermentation inoculum and its manufacture, can solve the problems of insufficient fermentation, low fermentation temperature, and incomplete killing of harmful organisms.

Inactive Publication Date: 2005-01-12
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the temperature of the fermentation heap is not high, the fermentation is not sufficient, and the harmful organisms are not completely killed.

Method used

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  • Zymogen agent for treating agricultural wastes and preparation method
  • Zymogen agent for treating agricultural wastes and preparation method
  • Zymogen agent for treating agricultural wastes and preparation method

Examples

Experimental program
Comparison scheme
Effect test

manufacture example

[0063] 1. Preparation of yeast strain ZJY2:

[0064] Saccharomyces spp. ZJY2 liquid shake flask medium: wort juice (10°Bx) 1000ml, glucose 18g, pH natural. Add 20 grams of agar with this as the medium to prepare a solid mother seed slant for inoculation and culture, take a loop of the slant mother seed and inoculate it in the medium of the yeast liquid shake flask (60ml medium / 250ml Erlenmeyer flask) according to the aseptic method , 28°C, rotation speed 180rpm, cultivated for 18 hours, the number of bacteria reached 0.5×10 8 More than cfu / ml is enough. Saccharomyces spp. solid fermentation culture medium: 99% of bran and a small amount of inorganic salt MgSO 4, NaCl each 0.05%, according to material, water ratio 1: 1 (w / w) add water to make material water content be about 60%. Take the liquid microbial agent cultivated in shake flasks as the bacterial classification, and evenly inoculate it in the solid fermentation material koji with an inoculation amount of 0.1% (the koj...

experiment example

[0083] The bacterial agent of the present invention is compared with Japanese enzyme bacteria, Beijing "organic waste fermentation fungus koji", and no inoculation. Using fresh pig manure as a raw material, adding 1.5% of rice bran and an appropriate amount of bacterial agent to carry out composting and fermentation comparative tests, the results are shown in the following table, The test site was in Xiaoshan City, Zhejiang Province, and the time was June 2003. The enzyme bacteria were purchased from Zhejiang Lvbao Enzyme Bioengineering Company, and the "organic waste fermentation koji" was purchased from Beijing Jingpuyuan Biological Engineering Co., Ltd.

[0084] Treatment Bacteria price Bacteria addition Fermentation days Moisture content Heap after 5 days Harmless Scale,

[0085] (yuan / ton) Amount added (days) Decrease (%) Temperature (℃) Chemical index Standardized production

[0086] (‰) Difficult to produce

[0087] Control 0 0 >30 <30 39 Hard...

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Abstract

A formentation bacterial agent for processing agricultural waste is composite or single bacterial agent prepared by cultivating and fermenting three bacterial agents of retaining cupsule film spoyeast ZJY2, griseolutein W, and cerecin ME1-2. The application process includes controlling water content rate of waste to be 55-56%, adding and mixing the agent, stirring the mixer for ventilation at setting time for 20-30 days and achieving the aim of making the waste nonhazardous and resources.

Description

technical field [0001] The invention belongs to the field of fermented bacterial agents for treating agricultural, animal husbandry and food processing wastes and the manufacture thereof, in particular to a bacterial agent capable of accelerating material fermentation, a preparation method of the bacterial agent and an application thereof. Background technique [0002] my country's large-scale livestock and poultry breeding industry has developed rapidly in the past ten years, especially the pig farming industry with a high degree of intensive farming has achieved considerable development, but it has also caused serious environmental pollution while improving production levels . In addition, a large amount of crop stalks are produced every year in agricultural production, as well as agricultural product processing wastes (such as skins, leaves, dregs, and edible fungus dregs, etc.), which aggravate rural environmental pollution. These livestock an...

Claims

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Application Information

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IPC IPC(8): B09B3/00C05F11/08C12N1/00C12N1/16C12N1/20
Inventor 薛智勇王卫平朱凤香钱江吴传珍何福恒章锦秋
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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