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Plant rhizosphere growth promoting bacteria agent production method using streptomycete

The technology of a growth-promoting fungicide and Streptomyces is applied in the directions of plant growth regulators, botanical equipment and methods, biocides, etc., to achieve the effects of short production cycle, convenient use and low cost

Inactive Publication Date: 2005-01-19
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, among all the products researched and developed, except for the embodiment applied by the applicant, no marine microorganisms have been used to manufacture PGPR preparations, and some characteristics of marine microorganisms, such as stress resistance, may be more beneficial Microbes function, especially in efficient competition with soil-indigenous microbes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The steps of utilizing Streptomyces flavinus to manufacture a plant rhizosphere growth-promoting agent are as follows:

[0021] 1) Activation of strains on inclined planes and cultivation of seeds in Kirschner flasks:

[0022] Firstly prepare the modified Gao's No. 1 medium for slant and seeded Kjeldahl flasks, the composition is KNO 3 1.0g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.5g, FeSO 4 ·7H 2 O 0.01g, 20.0g soluble starch, first heat with 200ml of water while stirring until the solution is transparent, make up water to 1000ml, adjust the pH to 7.0-7.2 with NaOH, add 20.0 (or 18.0) g of agar, and heat the above medium to Melt the agar completely, put about 10ml in each test tube, about 50ml in a Kjeldahl bottle, seal it with a cotton plug and wrap it with kraft paper, and sterilize at 121℃, 0.1MPa pressure for 20min;

[0023] After the sterilization is completed, when the culture medium is cooled to about 50°C, take out the test tube and the K-shaped bo...

Embodiment 2

[0033] The steps of utilizing Streptomyces roseus flavum to manufacture plant rhizosphere growth-promoting bacteria are as follows:

[0034] 1) slant-plane activation of Streptomyces roseus flavum strains and seed Kelvin flask cultivation are the same as in Example 1 (1);

[0035] 2) Seed culture medium and expanded fermentation culture of liquid fermenter for the above strains: 1M 3 Carbon steel universal fermenter, 700L of material, the weight composition of raw materials is 1.0% of corn flour, 1.0% of soybean cake powder, 0.5% of starch, K 2 HPO 4 0.01%; Soak 700ml of Die, the balance is tap water, pH 7.0 before disinfection, gauge pressure 0.1MPa 121 ℃ for 30min, cool to 30 ℃, insert 4 bottles of Kerry flask seeds, and sterilize with 300ml sterile water Wash the actinomycetes spores in the seed Kettler flask to make a bacterial suspension, at 28-30°C, the ventilation rate is 1:0.5 in volume / volume, mechanically stirred at 160-180r / min, and used as a seed tank for about ...

Embodiment 3

[0041] The steps of utilizing Streptomyces griseus to manufacture a plant rhizosphere growth-promoting agent are as follows:

[0042] 1) Streptomyces griseus bacterial classification slant activation and seed Kirschner flask culture are the same as embodiment 1 (1);

[0043] 2) liquid fermenter seed culture medium and expanded fermentation culture are the same as embodiment 2 (2);

[0044] 3) The above-mentioned fermented bacteria are subjected to solid adsorption disc mechanical granulation: on the disc of the disc granulator for the production of fertilizer granules, the pre-mixed materials are sprayed while granulating, and the Streptomyces bacteria liquid is sprayed The weight ratio with the solid material is 1:5, and the solid material is calculated in weight percent: 45% of brown powder, 10% of ammonium chloride, 40% of superphosphate, and 5% of sea foam;

[0045] 4) Low-temperature drum drying: The conveyor belt sends the preparation granules produced by the above-ment...

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Abstract

The invention relates to a plant rhizosphere growth promoting bacteria agent production method using streptomycete which comprises, (1) selecting streptomycete bacterial which has antagonistic action to plant pathogeny fungus and can be fixed into the crop rhizosphere soil, using farm and side line products as raw material and fermentation tank for submerged liquid fermentation, (2) subjecting the fermented streptomycete liquid to mixed granulation with solid material, and preparing the end product through low temperature drying.

Description

technical field [0001] The invention relates to an agricultural microbial biological agent, in particular to a method for producing a plant rhizosphere growth-promoting bacterium by using Streptomyces. Background technique [0002] There are various and active microorganisms in the rhizosphere of crops, which constitute a unique rhizosphere soil microbial flora, among which microorganisms that can promote plant growth, prevent diseases, and increase crop yields are called plant growth-promoting rhizobacteria (PGPR for short) ( Document 1: Kloepper and Schroth, 1978; Schippers, B., Bakker, A.W, Backker, P.A.H.M., Interactions of deleterious and beneficial rhizosphere microorganisms. Annu. Rev. Phytopathol25: 339-358, 1987), rhizosphere growth-promoting bacteria PGPR on soil Harmful pathogenic microorganisms and non-parasitic rhizosphere harmful microorganisms (Deleterious rhizosphere microorganisms, referred to as DRMO) have biocontrol effects, and can also promote the absorp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00C12N1/20
Inventor 胡江春薛德林张俊王伟马成新王书锦
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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