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Use of protein fingerprint method in detecting protein fingerprint of gastric carcinoma cell in blood

A protein and protein technology, which is applied in the field of protein fingerprinting and protein fingerprinting of biological sample analysis, can solve the problems of limited gel capacity and sensitivity, complexity, and inability to observe molecular weight.

Inactive Publication Date: 2005-03-16
BEIJING SELDI TECH
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

First, the method provides only two characteristics of biomolecules - mass and isoelectric point (pI)
Second, the resolution in each dimension is limited by the resolving power of the gel
For example, it is often difficult to distinguish between biomolecules that differ by less than 5% in mass or that differ in pI
Third, gels have limited capacity and sensitivity and may not be able to detect small amounts of expressed biomolecules
Fourth, small proteins or peptides with a molecular weight below about 10-20 kDa cannot be observed
Since human cell proteins are much more complex than potatoes, electrophoresis alone to identify protein fingerprints of diseases is far from meeting clinical needs

Method used

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  • Use of protein fingerprint method in detecting protein fingerprint of gastric carcinoma cell in blood
  • Use of protein fingerprint method in detecting protein fingerprint of gastric carcinoma cell in blood
  • Use of protein fingerprint method in detecting protein fingerprint of gastric carcinoma cell in blood

Examples

Experimental program
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Effect test

Embodiment 1

[0063] Example 1 Distinguishing Protein Fingerprints of Normal Human Cells and Gastric Adenocarcinoma Patient Cells in Blood

[0064] (1) Experimental method

[0065] 1. Materials

[0066] 1. Source of specimens: Serum samples of all patients were preoperative sampling specimens from patients with gastric adenocarcinoma confirmed by pathological examination after surgery, and control samples were from healthy volunteers with normal physical examination. Patients and healthy volunteers: 33 cases of gastric cancer (22 males, aged 44-80 years; 11 females, aged 64-71 years) and 31 age-matched non-gastric cancer healthy volunteers (20 males, aged 50-78 11 years old; 11 females, aged 60-75 years) serum samples were used for this test.

[0067] 2. Reagent: U9 buffer (weigh 27.027g of Urea, 2% CHAPS 1.0g, 1% DTT 0.5g to make the final capacity 50ml), U1 buffer (use 50mM Tris-HCl PH9 buffer, 9-fold dilution of U9 buffer solution into U1 buffer), cationic chip buffer (50-100mM Tris-H...

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Abstract

This invention also relates to protein dactylography method for analyzing for the protein group got by retention chromatography. This method adopts mass spectroscopy to distinguish and detect protein group. The analysis data takes the form of bar code (protein dactylography) read by computer. This invention method can be used to detect protein fingerprint in the normal cell and cancer of stomach patient cell in the blood.

Description

technical field [0001] The invention relates to a novel method for analyzing proteins in biological samples, in particular to a protein fingerprint method for analyzing biological samples. The present invention also relates to protein fingerprinting in which the proteome is captured by retention chromatography and analyzed using a computer readable barcode format (protein fingerprint). Background technique [0002] Both normal function and pathological characteristics of cells depend to some extent on the function of proteins expressed by cells. Therefore, identifying differences in proteins expressed within cells can be used in the diagnosis of disease and ultimately in drug development and disease treatment. However, differential analysis of protein expression and function requires the ability to resolve complex mixtures of intracellular molecules. However, many substances in cells often exist in trace amounts. The current methods for analyzing proteins have limitations ...

Claims

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Application Information

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IPC IPC(8): G01N30/00G01N30/72G01N33/53G01N33/68G06K7/10H01J49/04
Inventor 许洋梁勇
Owner BEIJING SELDI TECH
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