Method and use of producing soluble recombinant protein in colibacillus

Abstract

The invention was involved in that target protein expression strain and molecular partner were expressed in E.coli.at the same time at low temperature to prevent the formation of target protein inclusion body effectively and increase yields of soluble production largely. Soluble recombinant human endostatin and human angiostatin were increased largely by the technique. It provided a new simple and cheap technique to produce soluble recombinant protein with biological activity. It was applied in bioengineering pharmaceutical factory, gene engineering, biochemistry and molecular biology with high efficiency, cheap price, wide application and strong promotion.

Description

1. Technical field:

[0001] The invention belongs to the field of genetic engineering biotechnology. 2. Background technology:

[0002] The Escherichia coli expression system has the characteristics of fast growth, clear genetic background, high expression level and low cost, and is an ideal foreign protein expression system. However, the biggest disadvantage of this expression system is that foreign proteins often cannot be folded correctly and exist in the form of inactive inclusion bodies, and the yield of inclusion body refolding is usually very low. Finding a simple, easy, and versatile method to improve or prevent the formation of inclusion bodies is a difficulty and a bottleneck in the current E. coli expression system.

[0003] Angiogenesis is essential for the growth and metastasis of solid tumors. In order to promote angiogenesis, tumor tissue produces a series of angiogenic factors (such as bFGF), and many tumors also produce anti-angiogenic proteins, one of whic...

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