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Culturing method for sugarcane germ plasm

A breeding method and new germplasm technology, applied in the field of breeding sugarcane stress-resistant germplasm, can solve problems such as difficult flowering, complex genome structure, and different chromosome ploidy, and achieve increased yield and sugar content, short cultivation period, and low cost Effect

Inactive Publication Date: 2005-07-27
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI +1
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Problems solved by technology

[0003] Since the modern sugarcane cultivars are interspecific hybrids of the tropical species of Saccharum genus, Moshoumi and Indian species, during the long-term noble breeding process, the abnormality of meiosis makes the modern sugarcane cultivars form a large number of heterogeneous Ploidy, the number of chromosomes is large, the genome structure is complex, and the ploidy of chromosomes in the same plant is different. At the same time, because sugarcane is sensitive to photoperiod, many important parents are difficult to flower in subtropical and temperate regions. Even if the amount of pollen is insufficient, and often The flowering period of related plants is not met, which brings many difficulties to conventional stress resistance breeding and affects the progress of sugarcane stress resistance breeding

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  • Culturing method for sugarcane germ plasm

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Embodiment Construction

[0018] The present invention will be described in detail below in conjunction with examples.

[0019] A method for cultivating sugarcane stress-resistant new germplasm materials designed by the present invention, including the induction process of sugarcane embryogenic callus, the construction of the plant expression vector of the trehalose synthase gene of Grifola frondosa, and the process of transforming Agrobacterium, Agrobacterium It mediates the transformation of sugarcane embryogenic callus by the trehalose synthase gene, the cultivation process of transgenic plants, and the identification and screening process of stress-resistant materials.

[0020] 1. The induction process of sugarcane embryogenic callus: take the young and tender leaf base of excellent sugarcane cultivars as explants, and the explants are first sterilized with 75% ethanol on the surface and then sterilized with mercuric chloride solution, and then sterilized with mercuric chloride solution. Rinse with...

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Abstract

A method for culturing the new stress-resistant variety of sugar cane includes such steps as transferring the mycose synthetase gene into the enbryonic cells of sugar cane by agrobacterium mediation method and culturing the transgenic plant of sugar cane. Its advantages are short period, high efficiency and high stress-resistant effect.

Description

technical field [0001] The invention relates to a method for cultivating sugarcane stress-resistant germplasm by using plant genetic engineering technology. Background technique [0002] Sugarcane (Saccharum officinarum L.) is the most important sugar crop in the world, and it is also an important sugar crop in tropical and subtropical regions of my country. At present, my country is the third largest sugar-producing country in the world. In the 2003 / 2004 crush season, the total sugar production in the country reached 10.02 million tons, of which cane sugar accounted for 94% of the total sugar production. In recent years, sugarcane has also developed into an important energy crop and an important raw material for the production of green fuel-ethanol. my country's sugarcane is mainly planted in 13 provinces including Guangxi, Yunnan, Guangdong, Fujian, and Hainan. The planting area has exceeded 1.2 million hectares, of which dryland sugarcane area acc...

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Application Information

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IPC IPC(8): A01H1/00
Inventor 张树珍杨本鹏冯翠莲曾艳波罗敬萍蔡文伟杨学
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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