Injection of calf blood de-protein extract and preparation method thereof

A technology of calf blood and injection, applied in the field of injection, can solve the problems of many steps, loss of effective components, long time, etc., and achieve the effect of high biological activity

Inactive Publication Date: 2005-11-23
赵红梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, calf blood deproteinized extract is mainly prepared by concentrating calf blood, ultrafiltration or dialysi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Collect a total of 10 liters of venous blood from 6-month-old calves, put it in a sterilized wide-mouth plastic bucket, stir it clockwise with a rough-surfaced stirring paddle for 5 minutes, then slowly stir it for 10 minutes, lift the stirring paddle, drain the plasma, and remove Sticky clumps on the stirring paddle; add 30 grams of sodium citrate to the remaining plasma, stir well, refrigerate overnight at 4°C, skim off the surface oil film, put it into a centrifuge tube and centrifuge at 3000r / min for 15min, separate the blood cells, and use double Layer gauze filter out the upper layer of plasma to obtain about 9.5 liters; the plasma is passed through a beaded chitosan column (blood volume: chitosan amount is 10:1), and the flow rate is controlled at 3ml / min.cm 2 , wash the column with 500ml of distilled water until the effluent is colorless to obtain 10 liters of chromatographic solution; add 5 liters of 95% ethanol to the chromatographic plasma under stirring, stir...

Embodiment 2

[0019] Collect 15 liters of venous blood from a 6-month-old calf, put it in a sterilized jar, stir clockwise with a rough-surfaced paddle for 5 minutes, then slowly for 10 minutes, lift the paddle to drain the plasma, and remove the For sticky clumps, add 45 grams of sodium citrate to the remaining plasma and stir, refrigerate overnight at 4°C, skim off the oil film on the surface the next morning, put it into a centrifuge tube and centrifuge at 3000r / min for 15min, and cover with double-layer gauze Filter out the upper layer of plasma to obtain about 14.2 liters (retained blood cells), and pass the plasma through a beaded chitosan layered column (plasma volume: chitosan is 10:1), and the flow rate is controlled at 2ml / min.cm 2 , add 800ml of distilled water to wash the column from the top until the effluent is colorless, and 15 liters of layered liquid is obtained. Add 75 liters of 95% ethanol to the chromatographic plasma under stirring, stir for 15 minutes, put it into a ce...

Embodiment 3

[0021] Collect 20 liters of venous blood from a 6-month-old calf, put it in a sterilized jar, stir it with a rough-surfaced stirring paddle in the direction for 5 minutes, then slowly stir it for 10 minutes, lift the stirring paddle to control the blood, and discard the For the sticky mass, add 60 grams of sodium citrate to the blood plasma in the bottle and stir well, then refrigerate at 4°C for more than 12 hours, then remove the surface oil film, put it into a centrifuge tube, centrifuge at 3000r / min for 15 minutes, and filter with double gauze , the intercepted blood cells were discarded, and the supernatant plasma was about 19 liters, and the plasma was passed through a beaded chitosan column to adsorb foreign proteins (plasma volume: chitosan was 10: 1) and the flow rate was controlled at 1ml / min.cm 2 Take 1 liter of distilled water to wash the column until the effluent is colorless to obtain 20 liters of chromatographic solution. Then add 10 liters of 95% ethanol to the...

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PUM

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Abstract

The invention discloses an injection of calf blood de-protein extract and preparation method, wherein 1ml of the injection comprises solid 41-60mg, 55-65wt% of inorganics, 35-45wt% of organic active compound. The making process comprises the following steps, (1) stirring carf blood or serum, charging sodium citrate into the plasma, homogenizing and storing by refrigeration, separating and collecting plasma for later use, (2) passing the plasma through pearl form chitosan chromatography column, charging ethanol, stirring and centrifuging the protein precipitate, reclaiming ethanol from the supernatant fluid, charging proteinases, enzymolyzing, (3) charging distilled water into enzymolysis liquid, heating and filtering with chitosan modified activated charcoal column, (4) passing the filter liquor through modified activated charcoal column, passing through microporous filtration film under pressurization, loading, sealing, finally carrying out hot-pressed sterilization.

Description

technical field [0001] The invention relates to an injection, in particular to an injection of deproteinized calf blood extract and a preparation method thereof, belonging to the field of medicines. Background technique [0002] Calf blood deproteinized extract (serotonin) has been popularized and applied all over the world for 50 years since it was successfully developed by Sugao Pharmaceutical Factory in Switzerland in 1955. Its injection contains 40-50 mg calf blood deproteinized extract per milliliter, 30% of which are amino acids, sugars, ketoacids, purines, nucleotides and oligopeptides with a relative molecular weight of 2500-3000, 70% is an inorganic component. Calf blood deproteinized extract can significantly improve the activity of reticuloendothelial system, increase enzyme activity, accelerate cell oxidative phosphorylation reaction and ATP synthesis, activate cell respiration, promote metabolism, and accelerate tissue regeneration. Clinically, it is mainly us...

Claims

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Application Information

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IPC IPC(8): A61K35/14
Inventor 赵红梅
Owner 赵红梅
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