Determination of inorganic phosphorus and its diagnostic reagent kit
A diagnostic kit and inorganic phosphorus technology, applied in the field of medical testing and determination, can solve the problems of differences in the accuracy of sensitivity test results, affecting the use and promotion, etc.
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Embodiment 1
[0053] Embodiment one (single dose)
[0054] Prepare the diagnostic kit for inorganic phosphorus in serum according to the following components and dosage:
[0055] Glycine buffer 80mmol / l,
[0056] Inosine 1mmol / l,
[0057] Ethanol 3mmol / l,
[0058] thio-NAD + 1mmol / l,
[0059] Nucleoside phosphatase 5000U / l,
[0060] Xanthine oxidase 5000U / l,
[0061] Catalase 5000U / l,
[0062] Aldehyde dehydrogenase 5000U / l,
[0063] Ethylene glycol 50% (accounting for the total volume of the reagent).
[0064] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 37°C, reaction time 10 minutes, test main wavelength 340nm, test secondary wavelength 405nm or more, the volume ratio of the tested sample to the reagent is 1:25, and the reaction direction It is a positive reaction (absorbance rises, the same below).
[0065] Add the serum sample and the prepared single agent, and the two are automatically mixed in the analyzer to detect and record the in...
Embodiment 2
[0066] Embodiment two (two doses)
[0067] Prepare the diagnostic kit for inorganic phosphorus in serum according to the following components and dosage:
[0068] Reagent I——
[0069] Imidazole ~ hydrochloric acid buffer 100mmol / l,
[0070] Ethanol 8mmol / l,
[0071] NADP + 3mmol / l,
[0072] Nucleoside phosphatase 8000U / l,
[0073] Xanthine oxidase 8000U / l,
[0074] Catalase 8000U / l,
[0075] Aldehyde dehydrogenase 8000U / l,
[0076] Glycerol 50% (accounting for the total volume of reagent I);
[0077] Reagent II——
[0078] Imidazole ~ hydrochloric acid buffer 100mmol / l,
[0079] Inosine 3mmol / l,
[0080] Ethylene glycol 20mmol / l.
[0081] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 30°C, reaction time 15 minutes, test main wavelength 340nm, test secondary wavelength 405nm or more, the ratio of the total volume of the tested sample to reagent I and reagent II is 1:25, the ratio of the amount of reagent I to rea...
Embodiment 3
[0083] Embodiment three (three doses)
[0084] Prepare the diagnostic kit for inorganic phosphorus in serum according to the following components and dosage:
[0085] Reagent I——
[0086] Triethanolamine buffer 120mmol / l,
[0087] Ethanol 12mmol / l,
[0088] thio-NADP + 5mmol / l,
[0089] Propylene glycol 20mmol / l;
[0090] Reagent II——
[0091] Triethanolamine buffer 120mmol / l,
[0092] Nucleoside phosphatase 10000U / l,
[0093] Xanthine oxidase 10000U / l,
[0094] Catalase 10000U / l,
[0095] Aldehyde dehydrogenase 10000U / l,
[0096] Propylene glycol 50% (accounting for the total volume of reagent II);
[0097] Reagent III——
[0098] Triethanolamine buffer 120mmol / l,
[0099] Inosine 5mmol / l,
[0100] Ethylene glycol 20mmol / l.
[0101] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 25°C, reaction time 20 minutes, test main wavelength 340nm, test secondary wavelength 405nm or more, the total volume of the tested sample and reage...
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