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Determination of inorganic phosphorus and its diagnostic kit

A diagnostic kit and inorganic phosphorus technology are applied in the field of medical testing and determination, which can solve the problems affecting the use and promotion, the difference in the accuracy of the sensitivity test results, etc., and achieve the effects of low test cost, easy popularization and application, and accurate test results.

Inactive Publication Date: 2009-01-14
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with different reagents and testing routes, the sensitivity of detection and the accuracy of detection results will be very different, which directly affects the use and promotion of this method.

Method used

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  • Determination of inorganic phosphorus and its diagnostic kit
  • Determination of inorganic phosphorus and its diagnostic kit
  • Determination of inorganic phosphorus and its diagnostic kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment one (single agent)

[0046] Prepare the diagnostic kit for inorganic phosphorus in serum according to the following components and dosage:

[0047] Glycine buffer 80mmol / l,

[0048] Glyceraldehyde-3-phosphate 2mmol / l,

[0049] thio-NAD + 1mmol / l,

[0050] Glyceraldehyde-3-phosphate dehydrogenase 10000U / l,

[0051] Ethylene glycol 50% (accounting for the total volume of the reagent).

[0052] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 37°C, reaction time 10 minutes, test main wavelength 340nm, test secondary wavelength 405nm or more, the volume ratio of the tested sample to the reagent is 1:25, and the reaction direction It is a positive reaction (absorbance rises, the same below).

[0053] Add the serum sample and the prepared single agent, and the two are automatically mixed in the analyzer to detect and record the increase of the absorbance at 340nm. A total of 31 reading points were detected, and the data was r...

Embodiment 2

[0054] Embodiment two (two doses)

[0055] Prepare the diagnostic kit for inorganic phosphorus in serum according to the following components and dosage:

[0056] Reagent I——

[0057] Imidazole ~ hydrochloric acid buffer 100mmol / l,

[0058] Glyceraldehyde-3-phosphate 6mmol / l,

[0059] NADP + 2mmol / l,

[0060] Glycerin 20mmol / l

[0061] Reagent II——

[0062] Imidazole ~ hydrochloric acid buffer 100mmol / l,

[0063] Glyceraldehyde-3-phosphate dehydrogenase 20000U / l,

[0064] Ethylene glycol 50% (accounting for the total volume of reagent II).

[0065] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 30°C, reaction time 15 minutes, test main wavelength 340nm, test secondary wavelength 405nm or more, the ratio of the total volume of the tested sample to reagent I and reagent II is 1:25, the ratio of the amount of reagent I to reagent II is 4:1, and the reaction direction is positive reaction.

[0066] Serum sample and reagent I were add...

Embodiment 3

[0067] Embodiment three (three doses)

[0068] Prepare the diagnostic kit for inorganic phosphorus in serum according to the following components and dosage:

[0069] Reagent I——

[0070] Triethanolamine buffer 120mmol / l,

[0071] thio-NADP + 4mmol / l,

[0072] Propylene glycol 50% (accounting for reagent I total volume);

[0073] Reagent II——

[0074] Triethanolamine buffer 120mmol / l,

[0075] Glyceraldehyde-3-phosphate 10mmol / l,

[0076] Propylene glycol 50% (accounting for the total volume of reagent II);

[0077] Reagent III——

[0078] Triethanolamine buffer 120mmol / l,

[0079] Glyceraldehyde-3-phosphate dehydrogenase 30000U / l,

[0080] Ethylene glycol 50% (accounting for the total volume of reagent III).

[0081] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 25°C, reaction time 20 minutes, test main wavelength 340nm, test secondary wavelength 405nm or more, the total volume of the tested sample and reagent I, reagent II and r...

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Abstract

A method for determining inorganic phosphorus includes utilizing glyceraldehyde - 3 - phosphate dehydrogenase to react with glyceraldehyde - 3 - phosphate under activation of inorganic phosphorus in serum sample to make oxidized coenzyme be reduced coenzyme then detecting rise amplitude of absorbance at main wavelength of 340nm before and after reaction. A diagnostic kit is also disclosed.

Description

technical field [0001] The invention relates to a method for measuring inorganic phosphorus and a diagnostic kit prepared by using the method, belonging to the technical field of medical examination and measurement. Background technique [0002] About 87% of the phosphorus in the human body exists in the bones, and the phosphorus in the blood maintains a dynamic balance relationship with the phosphorus in the bones. On the other hand, there is a certain relationship between the concentration of calcium and phosphorus in the blood, and the product of the two is kept within a certain range. In milligrams, the product of the content of calcium and phosphorus in about 100 ml of blood plasma is 35-40 . In normal people, when blood calcium increases, blood phosphorus decreases, and vice versa. When the above product is greater than 40, calcium and phosphorus are deposited in the bone tissue in the form of bone salt; when it is greater than 70, metastatic calcification may occur;...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/84G01N21/31
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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