Testicle sperm freeze-storage and revival method

A sperm and testicular technology, applied in the field of testicular sperm cryopreservation and recovery, can solve the problems of low motility, low testicular sperm count, and failure of cryopreserved testicular sperm recovery, etc., and achieves the effect of improving flexibility and efficiency.

Inactive Publication Date: 2006-07-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Low sperm count and low motility in the testis. After routine cryopreservation and recovery, it is often difficult to find viable sperm for ICSI
The failure of cryopreserved testicular sperm recovery is a common problem in assisted reproductive clinics

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Put the seminiferous tubules taken out from the biopsy into a small amount of modified human fallopian tube fluid (mHTF), crush the seminiferous tubules with elbow forceps, blow and beat them evenly with a pipette, and prepare testicular sperm by discontinuous density gradient separation method, and put them into 10% polyvinylpyrrolidone (PVP) microdroplets in intracytoplasmic sperm injection (ICSI) dishes.

[0014] The zona pellucida was derived from donated eggs in first meiotic prophase, metaphase or unfertilized eggs. The zona pellucida is prepared by using a micromanipulation system on an inverted microscope hot stage. The egg cells are fixed with an egg-holding needle with an inner diameter of 15um, and a hole is made at the 3 o'clock position with an injection needle with an inner diameter of 5um, and the cytoplasm is aspirated with the injection needle , until the zona pellucida becomes empty. The zona pellucida stays in the modified human fallopian tube fluid ...

Embodiment 2

[0018] Referring to the method of Example 1, a total of 34 zona pellucida are used to freeze sperm, of which 8 zona pellucida freeze testicular sperm, as a reference, the other 12 zona pellucida freeze few weak sperm, and 14 zona pellucida freeze normal sperm . A total of 530 live sperm were injected into the zona pellucida. Before freezing, each zona pellucida contained 15.6 sperm on average. After recovery, an average of 12.3 live sperm and 1.8 dead sperm were recovered from each zona pellucida. The recovery rate of testicular sperm was 71.7%. The sperm recovery rate was 78.7%. See Table 1 for the results.

[0019] sperm type

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PUM

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Abstract

The invention discloses a cooling storage and resuscitation method of testicle sperm, which is characterized by the following: taking the exhausting cytoplasmic transparent tape as carrier; loading the biopsy testicle sperm in the sealed transparent tape; reducing the sperm loss; improving the sperm recovery ratio by 70 percent; aspirating the sperm under the microscopic operation in the ICSI directly; improving the flexibility of non-sperm patient to receive the auxiliary fertility treatment.

Description

technical field [0001] The invention belongs to the field of assisted reproduction, and relates to a method for freezing and resuscitating testicular sperm. Background technique [0002] Modern assisted reproductive technology enables patients with azoospermia to obtain a small amount of sperm through testicular biopsy and undergo intracytoplasmic sperm injection (Intracytoplasmic sperm injection, ICSI) treatment to obtain offspring. Since the pregnancy rate of each ICSI treatment cycle is 35%-40%, in order to obtain pregnancy, some patients need to repeat ICSI treatment several times. If cryopreservation of testicular sperm was not performed, a testicular biopsy was performed every cycle. However, testicular biopsy is a traumatic operation. Repeated operations not only bring additional psychological pressure and economic burden to patients, but also may cause testicular tissue fibrosis, making it more and more difficult to find healthy testicular parenchyma. More seriousl...

Claims

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Application Information

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IPC IPC(8): A61B17/425A61B19/00A01N1/02A61K35/12A61B90/00
Inventor 黄荷凤徐晨明
Owner ZHEJIANG UNIV
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