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Method for producing bioassay substrate by superposing two substrates one on another and bioassay substrate

A bioassay and substrate technology, applied in the field of DNA chips and other bioassay discs, can solve the problems of inefficient time, low detection accuracy, etc.

Inactive Publication Date: 2006-09-20
SONY CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the DNA chip technology also has serious technical problems, among which, the reaction takes a long time due to the low efficiency of hybridization, and the accuracy of detection is low due to the occurrence of false positives and false negatives.

Method used

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  • Method for producing bioassay substrate by superposing two substrates one on another and bioassay substrate
  • Method for producing bioassay substrate by superposing two substrates one on another and bioassay substrate
  • Method for producing bioassay substrate by superposing two substrates one on another and bioassay substrate

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Embodiment Construction

[0035] With reference to the accompanying drawings, the related preferred modes of the method according to the present invention suitable for the production of bioassay discs will be described below, and some preferred embodiments related to the bioassay discs will also be described.

[0036] first, figure 1 is an example for describing the basic structure of the bioassay disc according to the present invention, and a schematic diagram of the basic manufacturing method of the bioassay disc (1), and figure 2 is along figure 1 A schematic cross-sectional view in the direction of the arrow I-I (in a layered manner). exist figure 1 with figure 2 Reference numeral 1 shown in denotes a bioassay disc according to the present invention.

[0037] The bioassay disc 1 includes a lower first substrate designated by reference numeral 11 and an upper second substrate designated by reference numeral 12 . The bioassay substrate 1 can be manufactured by precisely aligning and laminating...

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Abstract

A bioassay substrate in which two pairs of opposed electrodes are provided in a reaction region, and high-order structure adjustment, movement, immobilization and so forth of a substance can freely be performed by applying a predetermined electric field to the electrodes. The bioassay substrate comprises first and second substrates (11, 12). The first substrate (11) is provided with a detection portion (X). The detection portion (X) has at least a reaction region (R) providing a field for interaction between substances and a first electrode (E11) facing the reaction region (R). The second substrate (12) has at least a second electrode (E12) for applying an electric field to the reaction region (R) between the electrodes (E11, E12). The two first and second substrates (11, 12) are superposed one on another so that the first and second electrodes (E11, E12) are opposed to each other. A method for producing such a bioassay substrate (1) is also disclosed.

Description

technical field [0001] The present invention relates to DNA chips and other bioassay disc technologies. More particularly, the present invention relates to a bioassay disc having opposing electrodes formed by aligning and laminating two electrode-carrying substrates together, and to a method of manufacturing the bioassay disc. Background technique [0002] The main background art related to the present invention will be discussed. The first background technology (conventional technology) is the integrated disc technology suitable for biological assays, which is generally called "DNA chip" or "DNA microarray" (hereinafter collectively referred to as "DNA chip") (see, for example, patent PCT Japanese translation of No. 4-505763 and PCT Japanese translation of Patent No. 10-503841). [0003] This type of DNA chip technology is characterized by the ability to perform comprehensive analysis of intermolecular reactions such as hybridization, because a plurality of oligonucleotid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N37/00C12M1/00G01N33/483G01N33/543
CPCG01N33/5438
Inventor 大西通博安芸祐一稲垣稔
Owner SONY CORP