Test paper for quick detection of cholera vibrio 01 group, 0139 group and cholera toxin

Abstract

The related test paper to fast detect comma bacillus 01 group, 0139 group and cholera toxin comprises: base on PVC plate carrier, from bottom to top, connecting a sample pad, a colloidal gold conjunct to overlay and label single antibody for former comma bacillus and toxin, the cellulose nitrate covered by other three monoclonal antibody on T1, T2 and T3 areas and anti-rat IG on C area, and an absorption pad. This invention has accurate result, strong specificity, and can play important role in early diagnosis for cholera.

Description

1. Technical field [0001] The invention relates to a test strip capable of rapidly detecting group 01, group 0139 and cholera toxin of vibrio cholerae at the same time by one operation. 2. Background technology [0002] Cholera is an acute intestinal infectious disease caused by Vibrio cholerae, which produces cholera toxin. Because of its sudden onset, rapid spread, and wide spread, cholera is listed as a Class A infectious disease by the Infectious Disease Law of my country. There are seven times in human history. The first cholera pandemic affected more than 100 countries and claimed the lives of tens of millions of people. Serogroup 01 is the main serogroup that caused these seven pandemics. In November 1992, a new, non-01 serogroup of Vibrio cholerae was discovered in India and Bangladesh, and was named 0139 serogroup. The 0139 group of Vibrio cholerae soon spread to more than a dozen countries and regions, and was It is predicted that a new world pandemic will likely f...

AI Technical Summary

Problems solved by technology

[0004] At present, the diagnosis method of cholera 01 group and 0139 group is mostly used to isolate and identify the pathogen. Although the specificity is strong, it takes a long time, usually more than ten hours, which is not conducive to timely and early diagnosis. There are also some conventional auxiliary diagnosis methods. Methods such as: serum agglutination test, brake test, etc., but these methods have problems such as poor specificity and low sensitivity. There is also a method with high sensitivity, namely polymerase chain reaction (PCR), but the instruments required for this method It is expensive and complicated to operate, and it is very easy to cause false positives due to operational poll

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